Rat Anti-OVA-IgE ELISA Kit | OVA sIgE elisa kit
Rat Anti-OVA-IgE ELISA Kit
Reactivity
Rat
Synonyms
Anti-OVA-IgE; N/A; Rat Anti-OVA-IgE ELISA Kit; OVA sIgE elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Anti-OVAIgE. No significant cross-reactivity or interference between Anti-OVAIgE and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Anti-OVAIgE and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive or Sandwich
Sensitivity
0.1?g/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for OVA sIgE elisa kit
Intended Uses: This Anti-OVAIgE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat Anti-OVAIgE. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: Anti-OVAIgE ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Anti-OVAIgE. Standards or samples are then added to the microtiter plate wells and Anti-OVAIgE if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Anti-OVAIgE present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Anti-OVAIgE are added to each well to "sandwich" the Anti-OVAIgE immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Anti-OVAIgE and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-OVAIgE concentration in each sample is interpolated from this standard curve.
Principle of the Assay: Anti-OVAIgE ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Anti-OVAIgE. Standards or samples are then added to the microtiter plate wells and Anti-OVAIgE if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Anti-OVAIgE present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Anti-OVAIgE are added to each well to "sandwich" the Anti-OVAIgE immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Anti-OVAIgE and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-OVAIgE concentration in each sample is interpolated from this standard curve.
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