Monkey Cortisol ELISA Kit | CORT elisa kit

Monkey Cortisol ELISA Kit

Cat. Num. AAA16762

• Reactivity: Monkey
• Synonyms: Cortisol; N/A; Monkey Cortisol ELISA Kit; CORT elisa kit

• Reactivity: Monkey
• Specificity: This assay has high sensitivity and excellent specificity for detection of CORT. No significant cross-reactivity or interference between CORT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CORT and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for CORT elisa kit

Principle of the Assay: CORT ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CORT antibody and an CORT-HRP conjugate. The assay sample and buffer are incubated together with CORT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CORT concentration since CORT from samples and CORT-HRP conjugate compete for the anti-CORT antibody binding site. Since the number of sites is limited, as more sites are occupied by CORT from the sample, fewer sites are left to bind CORT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CORT concentration in each sample is interpolated from this standard curve.

Intended Uses: This CORT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey CORT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!