Human glutaminase ELISA Kit | GLS elisa kit
Human Glutaminase kidney isoform, mitochondrial, GLS ELISA Kit
Gene Names
GLS; GAC; GAM; KGA; GLS1; AAD20
Reactivity
Human
Synonyms
glutaminase; N/A; Human Glutaminase kidney isoform, mitochondrial, GLS ELISA Kit; GLS elisa kit
Reactivity
Human
Assay Type
Sandwich
Detection Range
10 ng/mL-0.156 ng/mL
Sensitivity
Up to 0.05 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for GLS elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human GLS monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65,460 Da
NCBI Official Full Name
glutaminase kidney isoform, mitochondrial isoform 2
NCBI Official Synonym Full Names
glutaminase
NCBI Official Symbol
GLS
NCBI Official Synonym Symbols
GAC; GAM; KGA; GLS1; AAD20
NCBI Protein Information
glutaminase kidney isoform, mitochondrial; K-glutaminase; glutaminase C; L-glutamine amidohydrolase; glutaminase, phosphate-activated
UniProt Protein Name
Glutaminase kidney isoform, mitochondrial
UniProt Gene Name
GLS
UniProt Synonym Gene Names
GLS1; KIAA0838; GLS
UniProt Entry Name
GLSK_HUMAN
