FOXQ1 recombinant antibody
Rabbit anti-FOXQ1 Recombinant Monoclonal Antibody [BLR230K]
IP: 6 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval withTris-EDTA pH 9.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells.
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human FOXQ1 by western blot. Samples: Whole cell lysate (50 ug) from Hep-G2, HEK293T, NCI-H520, MUTZ-3, and HT-29 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FOXQ1 recombinant monoclonal antibody (AAA23877 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation)
(Detection of human FOXQ1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from NCI-H520 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-FOXQ1 recombinant monoclonal antibody (AAA23877 lot 1) used for IP at 6 ul/mg lysate. FOXQ1 was also immunoprecipitated by a second antibody against a different epitope of FOXQ1 (BL-2269A-2C5). For blotting immunoprecipitated FOXQ1, AAA23877 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human FOXQ1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Rabbit anti-FOXQ1 recombinant monoclonal antibody (AAA23877-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry)
(Detection of mouse FOXQ1 by immunohistochemistry. Sample: FFPE section of mouse bladder. Antibody: Rabbit anti-FOXQ1 recombinant monoclonal antibody (AAA23877-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human FOXQ1 by immunocytochemistry. Sample: FFPE section of NCI-H520 cells. Antibody: Rabbit anti-FOXQ1 recombinant monoclonal antibody (AAA23877-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)