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Mouse TAF11 Monoclonal Antibody | anti-TAF11 antibody
TAF11 (TAF11 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 28kD, MGC:15243, PRO2134, TAF2I, TAFII28) (Biotin)
Gene Names
TAF11; TAF2I; PRO2134; TAFII28; MGC:15243
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified
Synonyms
TAF11, Antibody; TAF11 (TAF11 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 28kD, MGC:15243, PRO2134, TAF2I, TAFII28) (Biotin); TAF11 RNA Polymerase II; TATA Box Binding Protein (TBP)-Associated Factor; 28kD; MGC:15243; PRO2134; TAF2I; TAFII28; anti-TAF11 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3H5
Specificity
Recognizes TAF11.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-TAF11 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
TAF11 (NP_005634, 158aa-210aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
SKVFVGEVVEEALDVCEKWGEMPPLQPKHMREAVRRLKSKGQIPNSKHKKIIF
Conjugate
Biotin
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
IHC (Immunohistchemistry)
(Immunoperoxidase of monoclonal antibody to TAF11 on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 1.2 ug/ml])
IHC (Immunohistochemistry)
(Immunoperoxidase of monoclonal antibody to TAF11 on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 1.2 ug/ml])
Application Data
(Detection limit for recombinant GST tagged TAF11 is approximately 0.1ng/ml as a capture antibody.)
IF (Immunofluorescence)
(Immunofluorescence of monoclonal antibody to TAF11 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence)
(Immunofluorescence of monoclonal antibody to TAF11 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot)
(TAF11 monoclonal antibody (M03), clone 3H5 Western Blot analysis of TAF11 expression in Hela S3 NE.)
Related Product Information for anti-TAF11 antibody
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a small subunit of TFIID that is present in all TFIID complexes and interacts with TBP. This subunit also interacts with another small subunit, TAF13, to form a heterodimer with a structure similar to the histone core structure. [provided by RefSeq]
Product Categories/Family for anti-TAF11 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23kDa
NCBI Official Full Name
transcription initiation factor TFIID subunit 11 isoform 1
NCBI Official Synonym Full Names
TATA-box binding protein associated factor 11
NCBI Official Symbol
TAF11
NCBI Official Synonym Symbols
TAF2I; PRO2134; TAFII28; MGC:15243
NCBI Protein Information
transcription initiation factor TFIID subunit 11
UniProt Protein Name
Transcription initiation factor TFIID subunit 11
UniProt Gene Name
TAF11
UniProt Synonym Gene Names
TAF2I
UniProt Entry Name
TAF11_HUMAN
