Monkey Lipocalin-Type Prostaglandin D Synthase ELISA Kit | L-PGDS elisa kit

Monkey Lipocalin-Type Prostaglandin D Synthase ELISA Kit

Cat. Num. AAA244351

• Gene Names: PTGDS; PDS; PGD2; PGDS; LPGDS; PGDS2; L-PGDS
• Reactivity: Monkey
• Synonyms: Lipocalin-Type Prostaglandin D Synthase; N/A; Monkey Lipocalin-Type Prostaglandin D Synthase ELISA Kit; L-PGDS elisa kit

• Reactivity: Monkey
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.

• Intended Uses: This LPGD2S ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey LPGD2S. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for L-PGDS elisa kit

Principle of the assay: LPGD2S ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LPGD2S antibody and an LPGD2S-HRP conjugate. The assay sample and buffer are incubated together with LPGD2S-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LPGD2S concentration since LPGD2S from samples and LPGD2S-HRP conjugate compete for the anti-LPGD2S antibody binding site. Since the number of sites is limited, as more sites are occupied by LPGD2S from the sample, fewer sites are left to bind LPGD2S-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LPGD2S concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 22090638
• NCBI GeneID: 374110
• Molecular Weight: 20,844 Da
• NCBI Official Full Name: lipocalin-type prostaglandin D synthase
• NCBI Official Synonym Full Names: prostaglandin D2 synthase 21kDa (brain)
• NCBI Official Symbol: PTGDS
• NCBI Official Synonym Symbols: PDS; PGD2; PGDS; LPGDS; PGDS2; L-PGDS
• NCBI Protein Information: prostaglandin-H2 D-isomerase
• UniProt Protein Name: Chondrogenesis associated lipocalin
• UniProt Gene Name: L-PGDS
• UniProt Entry Name: Q8QFM7_CHICK