DB (Dot Blot)
(Dot Blot: The figure below is the result of using 1ug/mL anti- Aβ42 clone CA9 10C11 to detect 10ng/dot Aβ42 or Aβ40. The antibody showed no cross-reactivity with Aβ40.)
Mouse Amyloid Beta Peptide 42 Monoclonal Antibody
mAb anti-Amyloid Beta Peptide 42
Reactivity
Human and other primates; mouse, rat
Applications
ELISA, Western Blot, Dot Blot
Purity
Protein G affinity purified
Synonyms
Amyloid Beta Peptide 42, Antibody; mAb anti-Amyloid Beta Peptide 42; Beta amyloid peptide 42; Mouse monoclonal antibody to C-terminal of amyloid beta peptide 42 (Abeta42); anti-Amyloid Beta Peptide 42 antibody
Host
Mouse
Reactivity
Human and other primates; mouse, rat
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
CA9 10C11
Specificity
This antibody recognizes the C-terminal sequence (MVGGVVIA) of Aβ42 and full length Aβ42.
Purity/Purification
Protein G affinity purified
Form/Format
Lyophilized from a solution in 0.01M PBS, pH 7.2
Applicable Applications for anti-Amyloid Beta Peptide 42 antibody
Sandwich ELISA (S-EIA), Western Blot (WB), Dot Blot (DB)
Application Notes
Sandwich ELISA: In combination with anti-amyloid peptide N-terminus capture antibody (mAb clone NT 5B8, Cat. No.: ), the antibody can detect Aβ42 in sandwich ELISA assay.
Western blot: The figure below is the result of using 2ug/mL anti- Aβ42 clone CA9 10C11 to detect Aβ42 on Western blot following Tris-Tricine separation gel electrophoresis. The antibody showed no cross-reactivity with Aβ40.
Western blot: The figure below is the result of using 2ug/mL anti- Aβ42 clone CA9 10C11 to detect Aβ42 on Western blot following Tris-Tricine separation gel electrophoresis. The antibody showed no cross-reactivity with Aβ40.
Target Protein
C-terminus of amyloid beta peptide 42
Immunogen
KLH conjugated to a short peptide (MVGGVVIA) with amino acid sequence corresponding to the C-terminus of Aβ42
Fusion Myeloma
Sp2/0-Ag14
Reconstitution
Double distilled water is recommended to adjust the final concentration to 1.00mg/mL.
Product Type
Primary antibody, detection antibody in matched antibody pair
Research Area
Aging and neurodegenerative diseases
Cross-Reactivity
The antibody does not cross react with amyloid beta peptide 40 in dot blotting and ELISA. Cross-reactivity to amyloid beta peptide 43 is less than 1% in ELISA.
Preparation and Storage
Store at -20°C.
DB (Dot Blot)
(Dot Blot: The figure below is the result of using 1ug/mL anti- Aβ42 clone CA9 10C11 to detect 10ng/dot Aβ42 or Aβ40. The antibody showed no cross-reactivity with Aβ40.)
DB (Dot Blot)
(Dot Blot: The figure below is the result of using 1ug/mL anti- Aβ42 clone CA9 10C11 to detect 10ng/dot Aβ42 or Aβ40. The antibody showed no cross-reactivity with Aβ40.)
WB (Western Blot)
(Sandwich ELISA: In combination with anti-amyloid peptide N-terminus capture antibody (mAb clone NT 5B8, Cat. No.: please inquire), the antibody can detect Aβ42 in sandwich ELISA assayWestern Blot: The figure below is the result of using 2ug/mL anti- Aβ42 clone CA9 10C11 to detect Aβ42 on Western blot following Tris-Tricine separation gel electrophoresis. The antibody showed no cross-reactivity with Aβ40.Lane 1 &2 : Aβ40 Lane 3&4: Aβ42)
WB (Western Blot)
(Sandwich ELISA: In combination with anti-amyloid peptide N-terminus capture antibody (mAb clone NT 5B8, Cat. No.: please inquire), the antibody can detect Aβ42 in sandwich ELISA assayWestern Blot: The figure below is the result of using 2ug/mL anti- Aβ42 clone CA9 10C11 to detect Aβ42 on Western blot following Tris-Tricine separation gel electrophoresis. The antibody showed no cross-reactivity with Aβ40.Lane 1 &2 : Aβ40 Lane 3&4: Aβ42)
Related Product Information for anti-Amyloid Beta Peptide 42 antibody
Description:Description: Mouse monoclonal antibody to C-terminus of amyloid beta peptide 42 (Aβ42).
BackgroundAmyloid beta peptide 42 (Aβ42) is best known for its role in the formation of senile plaques in the brain of patients with Alzheimer's disease. Aβ42 and Aβ40 are the two major amyloid peptides that are produced after cleavage of amyloid precursor protein by secretases. Aβ42 (42 amino acids) is very fibrillogenic. The beta pleated structure of Aβ42 consitituents the intial and key component of the insoluble amyloid fibril in senile plaque. It is widely accepted that Aβ42 contibutes to the pathogenesis of Alzheimer's. One propsition is that the deposition of amyloid fibril onto the brain tissues results in Alzheimer's disease. Another is that the neurotoxicity of Aβ42 oligomer is the cause of the disease.
BackgroundAmyloid beta peptide 42 (Aβ42) is best known for its role in the formation of senile plaques in the brain of patients with Alzheimer's disease. Aβ42 and Aβ40 are the two major amyloid peptides that are produced after cleavage of amyloid precursor protein by secretases. Aβ42 (42 amino acids) is very fibrillogenic. The beta pleated structure of Aβ42 consitituents the intial and key component of the insoluble amyloid fibril in senile plaque. It is widely accepted that Aβ42 contibutes to the pathogenesis of Alzheimer's. One propsition is that the deposition of amyloid fibril onto the brain tissues results in Alzheimer's disease. Another is that the neurotoxicity of Aβ42 oligomer is the cause of the disease.
Product Categories/Family for anti-Amyloid Beta Peptide 42 antibody
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Product Notes
The Amyloid Beta Peptide 42 (Catalog #AAA14627) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The mAb anti-Amyloid Beta Peptide 42 reacts with Human and other primates; mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's Amyloid Beta Peptide 42 can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (S-EIA), Western Blot (WB), Dot Blot (DB). Sandwich ELISA: In combination with anti-amyloid peptide N-terminus capture antibody (mAb clone NT 5B8, Cat. No.: ), the antibody can detect Aβ42 in sandwich ELISA assay. Western blot: The figure below is the result of using 2ug/mL anti- Aβ42 clone CA9 10C11 to detect Aβ42 on Western blot following Tris-Tricine separation gel electrophoresis. The antibody showed no cross-reactivity with Aβ40. Researchers should empirically determine the suitability of the Amyloid Beta Peptide 42 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Amyloid Beta Peptide 42, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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