COVID 19 Nucleocapsid (NP) Coronavirus Antibody Pair Kit | COVID-19 antibody pair kit

Paired monoclonal antibodies to COVID-19 nucelocapsid

Cat. Num. AAA14650

• Applications: Lateral Flow
• Purity: Purified by Protein A affinity column
• Synonyms: COVID 19 Nucleocapsid (NP) Coronavirus, Antibody Pair Kit; Paired monoclonal antibodies to COVID-19 nucelocapsid; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Nucleocapsid; Nucleoprotein (NP); COVID-19 antibody pair kit

• Host: Mouse
• Clonality: Monoclonal
• Clone Number: Covid-Mab3, Covid-Mab4
• Purity/Purification: Purified by Protein A affinity column
• Form/Format: Purified, Liquid; PBS, pH 7.4 (with 0.1 % NaN₃)
• Concentration: A) Covid-Mab3: 10.85 mg/ml (COV2210208); B) Covid-Mab4: 11.4 mg/ml (COV2210416) (varies by lot)
• Quantity: 2 mg (1 mg each antibody packaged separately: 1 mg Clone Covid-Mab3 packaged in one vial and 1 mg Clone Covid-Mab4 packaged in a separate vial. Total: Two vials each containing 1 mg of antibody.)
• Clone Notes: Covid-Mab3, Covid-Mab4: These two monoclonal antibodies constitute a pair of monoclonal antibodies designed for developing lateral flow assays, specifically rapid antigen tests to detect nucleocapsid protein.

• Applicable Applications for COVID-19 antibody pair kit: Lateral Flow Assay
• Application Notes: Lateral flow assays. Clone Covid-Mab3 is suggested as the coating/capture antibody. Clone Covid-Mab4 is suggested as the conjugation antibody. However, researchers should optimize the pairing strategy according to their model system.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Store at -20°C. Avoid repeated freeze/thaw cycles.

Related Product Information for COVID-19 antibody pair kit

COVID-19 (coronavirus) also known as: SARS-CoV-2, 2019 Novel Coronavirus or Wuhan Coronavirus

Reference Data: The information in this section is for reference only. Covid-Mab3 has been used as the capture/coating antibody over the membrane at 2.2 mg/ml. Covid-Mab4 has used as the conjugation antibody conjugated to colloid. However, each laboratory should develop their own protocols based on their experience and expertise.

A laboratory study using the paired Covid-Mab3 and Covid-Mab4 antibodies showed the antibodies detected a low level of recombinant Covid 19 nucleocapsid protein (Cat no. MBS596190) down to 180pg/ml. Lowest detection (LoD) of TCID50/ml for inactivated SARS-CoV-2 virus WA1/2020 (NR-52286) from was 6.6 x 10³; this number is comparable to some COVID antigen rapid tests approved by the FDA. In this study, level of recombinant Covid 19 nucleocapsid protein (Cat no MBS596190) down to 180pg/ml. Lowest detection (LoD) of TCID50/ml for inactivated SARS-CoV-2 virus WA1/2020 (NR-52286) from was 6.6 x 103; this number is comparable to some COVID antigen rapid tests approved by the FDA. In this study, Two independent clinical studies were conducted using Clones Covid-Mab3 and Covid-Mab4 in rapid antigen tests. One clinical study was conducted in the Philippines, USA, and India and included 193 infected COVID-19 patients. The results of this study showed 91.6% sensitivity for infected patients and 100% specificity for 903 peoples without the infection. The rapid antibody test used in this study has been approved by CE marker and Health Canada.

The second clinical study was conducted in Nigeria and included 166 infected COVID-19 patients. The results of this study showed 89.5% sensitivity for infected patients, and 98.5% specificity for 67 uninfected individuals.

An additional study using Clones Covid-Mab3 and Covid-Mab4 in rapid antigen tests to detect the Omicron variant was conducted between January and February 2022 in Canada. The showed 100% positive for 25 Omicron patients and 100% specificity for the 50 uninfected people in Canada.