Application Data
(AAA13666 (1.5ug/ml) as the reporter with EB002025 as the capture rabbit antibody (5ug/ml).)
Goat FTL Polyclonal Antibody | anti-FTL antibody
Goat anti-FTL Antibody
Gene Names
FTL; LFTD; NBIA3
Reactivity
Tested: Human, Mouse, RatExpected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
ELISA, Western Blot, Immunohistochemistry, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
FTL, Antibody; Goat anti-FTL Antibody; Goat Anti-FTL, Biotinylated Antibody; FTL; ferritin, light polypeptide; MGC71996; L apoferritin; ferritin L subunit; ferritin L-chain; ferritin light chain; ferritin light polypeptide-like 3; FTL antibody; ferritin; light polypeptide antibody; MGC71996 antibody; L apoferritin antibody; ferritin L subunit antibody; ferritin L-chain antibody; ferritin light chain antibody; ferritin light polypeptide-like 3 antibody; anti-FTL antibody
Host
Goat
Reactivity
Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
GEYLFERLTLKHD
Sequence Length
175
Applicable Applications for anti-FTL antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 20kDa band observed in Human Brain (Cerebellum), Liver, Placenta and 22kDa in Mouse and Rat Brain lysates (calculated MW of 20.0kDa according to NP_000137.2). Recommended concentration: 0.3-1.0ug/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Spleen. Recommended concentration: 4-6ug/ml.
Western blot: Approx. 20kDa band observed in Human Brain (Cerebellum), Liver, Placenta and 22kDa in Mouse and Rat Brain lysates (calculated MW of 20.0kDa according to NP_000137.2). Recommended concentration: 0.3-1.0ug/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Spleen. Recommended concentration: 4-6ug/ml.
Immunogen
Peptide with sequence C-GEYLFERLTLKHD, from the C Terminus of the protein sequence according to NP_000137.2.
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Application Data
(AAA13666 (1.5ug/ml) as the reporter with EB002025 as the capture rabbit antibody (5ug/ml).)
Application Data
(AAA13666 (1.5ug/ml) as the reporter with EB002025 as the capture rabbit antibody (5ug/ml).)
IHC (Immunohistochemistry)
(AAA13666 Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.)
IHC (Immunohistochemistry)
(AAA13666 Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.)
IHC (Immunohistochemistry)
(AAA13666 (4ug/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.)
IHC (Immunohistochemistry)
(AAA13666 (4ug/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
ferritin light chain
NCBI Official Synonym Full Names
ferritin, light polypeptide
NCBI Official Symbol
FTL
NCBI Official Synonym Symbols
LFTD; NBIA3
NCBI Protein Information
ferritin light chain
UniProt Protein Name
Ferritin light chain
UniProt Gene Name
FTL
UniProt Synonym Gene Names
Ferritin L subunit
UniProt Entry Name
FRIL_HUMAN
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Product Notes
The FTL ftl (Catalog #AAA13666) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-FTL Antibody reacts with Tested: Human, Mouse, Rat Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's FTL can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx. 20kDa band observed in Human Brain (Cerebellum), Liver, Placenta and 22kDa in Mouse and Rat Brain lysates (calculated MW of 20.0kDa according to NP_000137.2). Recommended concentration: 0.3-1.0ug/ml. Primary incubation was 1 hour. IHC: Paraffin embedded Human Spleen. Recommended concentration: 4-6ug/ml. Researchers should empirically determine the suitability of the FTL ftl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GEYLFERLTL KHD. It is sometimes possible for the material contained within the vial of "FTL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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