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WB (Western Blot)
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Rabbit anti-Human, Mouse RPA32 Polyclonal Antibody | anti-RPA32 antibody

Rabbit anti-RPA32 Antibody, Affinity Purified

Gene Names
RPA2; REPA2; RPA32
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Antigen Affinity Purified
Synonyms
RPA32, Antibody; Rabbit anti-RPA32 Antibody, Affinity Purified; replication factor A protein 2; RPA32; RP-A p34; RP-A p32; RF-A protein 2; replication protein A 32 kDa subunit; REPA2; replication protein A 34 kDa subunit; Replication protein A 32 kDa subunit; replication protein A2; anti-RPA32 antibody
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For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-RPA32 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:2000 to 1:10,000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
Immunogen
Between 220 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse RPA32 by western blot. Samples: Whole cell lysate (10 ug) from CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPA32 antibody (AAA23770 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

WB (Western Blot)

(Detection of human RPA32 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HeLa, Jurkat, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPA32 antibody (AAA23770 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)

IP (Immunoprecipitation)

(Detection of human RPA32 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells treated with 100 uM etoposide for 4 hours (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-RPA32 antibody (AAA23770 lot 4) used for IP at 6 ug per reaction. RPA32 was also immunoprecipitated by rabbit anti-Phospho RPA32 (S4/S8) antibody . For blotting immunoprecipitated RPA32, AAA23770 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)

IHC (Immunohistochemistry)

(Detection of mouse RPA32 by immunohistochemistry. Sample: FFPE section of mouse kidney. Antibody: Affinity purified rabbit anti-RPA32 (AAA23770 Lot 4) used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB)

IHC (Immunohistochemistry)

(Detection of human RPA32 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RPA32 (AAA23770 Lot 4) used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB)

FCM (Flow Cytometry)

(Detection of human RPA32 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-RPA32 polyclonal antibody (AAA23770) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

Related Product Information for anti-RPA32 antibody
Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32kDa (RPA2), and 14kDa (RPA3). RPA2, the 32kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK.
Product Categories/Family for anti-RPA32 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,247 Da
NCBI Official Full Name
replication protein A 32 kDa subunit
NCBI Official Synonym Full Names
replication protein A2, 32kDa
NCBI Official Symbol
RPA2
NCBI Official Synonym Symbols
REPA2; RPA32
NCBI Protein Information
replication protein A 32 kDa subunit; RP-A p32; RP-A p34; RF-A protein 2; replication factor A protein 2; replication protein A 34 kDa subunit
UniProt Protein Name
Replication protein A 32 kDa subunit
UniProt Gene Name
RPA2
UniProt Synonym Gene Names
REPA2; RPA32; RPA34; RP-A p32; RF-A protein 2; RP-A p34
UniProt Entry Name
RFA2_HUMAN

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Product Notes

The RPA32 rpa2 (Catalog #AAA23770) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-RPA32 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RPA32 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:2000-1:10,000 IP: 6ug/mg lysate IHC: 1:2000 to 1:10,000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the RPA32 rpa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPA32, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.