Insulin Like Growth Factor 1 (IGF1) Active Protein | IGF1 active protein

Active Insulin Like Growth Factor 1 (IGF1)

Cat. Num. AAA146604

• Gene Names: IGF1; MGF; IGFI; IGF-I
• Applications: Activity Assay, Cell Culture
• Purity: > 95%
• Synonyms: Insulin Like Growth Factor 1 (IGF1); N/A; Active Insulin Like Growth Factor 1 (IGF1); IGFI; IGF1A; IBP1; MGF; Somatomedin C; Mechano Growth Gactor; IGF1 active protein

• Host: 293F cell
• Purity/Purification: > 95%
• Form/Format: Freeze-dried powder
• Sequence Length: 153

• Applicable Applications for IGF1 active protein: Activity Assay, Cell Culture
• Fragment: Gly49~Ala118
• Tag: N-terminal His Tag
• Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Organism Species: Human
• Isoelectric Point: 7.8
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Application Data

Application Data

Application Data

SDS-PAGE

Activity

(Insulin-like growth factor I (IGF1), is a hormone similar in molecular structure to insulin but have a much higher growth-promoting activity, it belongs to a family of proteins involved in mediating growth and development. It is reported that IGF1 induces the proliferation, migration, differentiation of a large types of cells including the MCF-7 breast cancer cell line. To test the effect of growth factors on proliferation, MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of IGF-1. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37 degree C.Cell proliferation of MCF-7 cells after incubation with IGF1 for 72h observed by inverted microscope was shown in Figure 1.)

Product Categories/Family for IGF1 active protein

Active Protein

NCBI and Uniprot Product Information

• NCBI GI #: 11024682
• NCBI GeneID: 3479
• NCBI Accession #: NP_000609.1
• NCBI GenBank Nucleotide #: NM_000618.4
• UniProt Accession #: P05019; P01343; Q14620; B2RWM7; E9PD02
• Molecular Weight: 9.3kDa; 14kDa
• NCBI Official Full Name: insulin-like growth factor I isoform 4 preproprotein
• NCBI Official Synonym Full Names: insulin like growth factor 1
• NCBI Official Symbol: IGF1
• NCBI Official Synonym Symbols: MGF; IGFI; IGF-I
• NCBI Protein Information: insulin-like growth factor I
• UniProt Protein Name: Insulin-like growth factor I
• UniProt Gene Name: IGF1
• UniProt Synonym Gene Names: IBP1; IGF-I; MGF