Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Functional Studies (can be used for cytotoxicity assays for alpha-Gal specific pathways) Other applications not tested
IF (Immunofluorescence) (Immunofluorescence of HeLa cells using 126766 (10ug/ml).)
IHC (Immunohistochemistry) (Immunohistochemistry of formalin-fixed paraffin-embedded human tonsil using 1267661 (3ug/ml) and Immunoperoxidase.)
IP (Immunoprecipitation) (Immunoprecipitation of FGF1 transfected lysate using 126766 and Protein A Magnetic Bead and immunoblotted with FGF1 rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of FGF1 expression in transfected 293T cell line using 126766. Lane 1: transfected lysate (17.5kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged FGF1 is ~10ng/ml using 126766 as a capture antibody.)
WB (Western Blot) (CHEK2 monoclonal antibody Western Blot analysis of CHEK2 expression in HeLa NE.)
Application Data (Detection limit for recombinant GST tagged CHEK2 is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CHEK2 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CHEK2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
WB (Western Blot) (CHEK2 monoclonal antibody Western Blot analysis of CHEK2 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (85.84kD).)
WB (Western Blot) (TGIF2 monoclonal antibody, Western Blot analysis of TGIF2 expression in HeLa NE.)
WB (Western Blot) (TGIF2 monoclonal antibody. Western Blot analysis of TGIF2 expression in SW-13.)
WB (Western Blot) (Western blot analysis of TGIF2 over-expressed 293 cell line, cotransfected with TGIF2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TGIF2 monoclonal antibody (M01). GAPDH (36.1kD). used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged TGIF2 is ~0.3ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of TGIF2 expression in transfected 293T cell line by TGIF2 monoclonal antibody. Lane 1: TGIF2 transfected lysate (25.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (TGIF2 monoclonal antibody. Western Blot analysis of TGIF2 expression in IMR-32.)
WB (Western Blot) (Western Blot detection against Immunogen (37.77kD).)
IP (Immunoprecipitation) (Immunoprecipitation of PSMA7 transfected lysate using PSMA7 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PSMA7 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PSMA7 on HeLa cell. [antibody concentration 1 ~ 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 5ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human lung, adenosqumous cell carcinoma. [antibody concentration 5ug/ml])
WB (Western Blot) (Western Blot analysis of PSMA7 expression in transfected 293T cell line by PSMA7 monoclonal antibody. Lane 1: PSMA7 transfected lysate (Predicted MW: 27.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PSMA7 monoclonal antibody Western Blot analysis of PSMA7 expression in HeLa.)
WB (Western Blot) (PSMA7 monoclonal antibody. Western Blot analysis of PSMA7 expression in human omentum, serous carcinoma.)
WB (Western Blot) (AKT1 monoclonal antibody Western Blot analysis of AKT1 expression in NIH/3T3.)
WB (Western Blot) (Western Blot analysis of AKT1 expression in transfected 293T cell line by AKT1 monoclonal antibody. Lane 1: AKT1 transfected lysate (55.7kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged AKT1 is ~3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of AKT1 transfected lysate using AKT1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with AKT1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AKT1 on HeLa cell. [antibody concentration 20ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to AKT1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot detection against Immunogen (78.54kD).)
WB (Western Blot) (TIMM9 monoclonal antibody. Western Blot analysis of TIMM9 expression in Raw 264.7.)
WB (Western Blot) (TIMM9 monoclonal antibody Western Blot analysis of TIMM9 expression in IMR-32.)
Application Data (Detection limit for recombinant GST tagged TIMM9 is ~0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMM9 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of TIMM9 expression in transfected 293T cell line by TIMM9 monoclonal antibody. Lane 1: TIMM9 transfected lysate (10.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (TIMM9 monoclonal antibody. Western Blot analysis of TIMM9 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (35.9kD).)
WB (Western Blot) (Detection of human OX40/CD134 by western blot. Samples: Whole cell lysate (50 ug) from HH, HeLa, MJ, HEK293T, Hep-G2, C5/MJ, Jurkat, and MOLT-4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal antibody (AAA23810 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Rabbit anti-eEF2 .)
IP (Immunoprecipitation) (Detection of human OX40/CD134 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-OX40/CD134 recombinant monoclonal antibody (AAA23810 lot 1) used for IP at 20 ul per reaction. OX40/CD134 was also immunoprecipitated by rabbit anti-OX40/CD134 recombinant monoclonal antibody [548-1D12]. For blotting immunoprecipitated OX40/CD134, AAA23810 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry) (Detection of human OX40/CD134 (red) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal antibody (AAA23810 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry) (Detection of human OX40/CD134 by immunohistochemistry. Sample: FFPE section of lung carcinoma. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal antibody (AAA23810 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human OX40/CD134 by immunocytochemistry. Sample: FFPE section of MJ cells. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal antibody (AAA23810 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry) (Detection of human OX40/CD134 (shaded) in MJ[G11] cells by flow cytometry. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal (AAA23810 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
WB (Western Blot) (FLJ23356 monoclonal antibody. Western Blot analysis of FLJ23356 expression in HeLa.)
WB (Western Blot) (FLJ23356 monoclonal antibody. Western Blot analysis of FLJ23356 expression in HepG2.)
Application Data (Detection limit for recombinant GST tagged FLJ23356 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FLJ23356 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of FLJ23356 expression in transfected 293T cell line by FLJ23356 monoclonal antibody.Lane 1: FLJ23356 transfected lysate (38.5kD).Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of FLJ23356 over-expressed 293 cell line, cotransfected with FLJ23356 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with FLJ23356 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between AKT1 and HSPB1. Mahlavu cells were stained with AKT1 rabbit purified polyclonal 1:1200 and HSPB1 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged HSPB1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of HSPB1 transfected lysate using HSPB1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HSPB1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HSPB1 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of HSPB1 expression in transfected 293T cell line by HSPB1 monoclonal antibody. Lane 1: HSPB1 transfected lysate (22.8kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot analysis of AFP expression in transfected 293T cell line by AFP monoclonal antibody. Lane 1: AFP transfected lysate (69kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged AFP is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of AFP transfected lysate using AFP monoclonal antibody and Protein A Magnetic Bead and immunoblotted with AFP rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AFP on HepG2 cell. [antibody concentration 30ug/ml].)
WB (Western Blot) (AFP monoclonal antibody Western Blot analysis of AFP expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
WB (Western Blot) (Western blot analysis of BIRC5 over-expressed 293 cell line, cotransfected with BIRC5 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with BIRC5 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged BIRC5 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of BIRC5 transfected lysate using BIRC5 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with BIRC5 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to BIRC5 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of BIRC5 expression in transfected 293T cell line by BIRC5 monoclonal antibody. Lane 1: BIRC5 transfected lysate (16.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to FOXA2 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 0.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA2 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 0.5 ug/ml])
Application Data (Detection limit for recombinant GST tagged FOXA2 is approximately 0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA2 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (FOXA2 monoclonal antibody (M12), clone 6C12 Western Blot analysis of FOXA2 expression in K-562.)
ICC (Immunocytochemistry) (ICC staining GST3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining GST3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining GST3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-GST3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-GST3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GST3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-GST3 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of GST3 on mouse liver tissue (1) and A549 cell (2) lysate using anti-GST3 antibody at 1/500 dilution.)
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC)
Purity
ProA affinity purified
Pricing
ELISA (Sandwich ELISA analysis of human PDGF-BB using Mouse anti Human PDGF-BB (AAA12291) as a capture reagent and biotinylated Mouse anti Human PDGF-BB as a detection reagent with purified recombinant PDGF-BB as the antigen. Detection was performed using HRP conjugated streptavidin and substrate.)
WB (Western Blot) (Detection of human B7-H4 by western blot. Samples: Whole cell lysate (50 ug) from OVCAR-4, Hep-G2, MCF-7, HeLa, HEK293T, Jurkat, MOLT-4, HH, and C5/MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-B7-H4 recombinant monoclonal antibody (AAA23811 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation) (Detection of human B7-H4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from OVCAR-4 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-B7-H4 recombinant monoclonal antibody (AAA23811 lot 1) used for IP at 20 ul per reaction. B7-H4 was also immunoprecipitated by rabbit anti-B7-H4 recombinant monoclonal antibody [209-2B5]. For blotting immunoprecipitated B7-H4, AAA23811 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry) (Detection of human B7-H4 (red) by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Rabbit anti-B7-H4 recombinant monoclonal antibody (AAA23811 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry) (Detection of human B7-H4 by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Rabbit anti-B7-H4 recombinant monoclonal antibody (AAA23811 lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human B7-H4 by immunocytochemistry. Sample: FFPE section of OVCAR-3 cells. Antibody: Rabbit anti-B7-H4 recombinant monoclonal antibody (AAA23811 lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry) (Detection of human B7-H4 (shaded) in human peripheral blood mononuclear cells by flow cytometry. Antibody: Rabbit anti-B7-H4 recombinant monoclonal (AAA23811 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Application Data (Detection limit for recombinant GST tagged PGR is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PGR on MCF-7 cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody, 131852, to PGR on formalin-fixed paraffin-embedded human breast cancer. [antibody concentration 3ug/ml])
WB (Western Blot) (PGR monoclonal antibody, 131852, Western Blot analysis of PGR expression in MCF-7.)
WB (Western Blot) (PGR monoclonal antibody, 131852, Western Blot analysis of PGR expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen, 131852 (37.84kD))
WB (Western Blot) (Western blot analysis of TCF19 over-expressed 293 cell line, cotransfected with TCF19 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TCF19 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged TCF19 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TCF19 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TCF19 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of TCF19 expression in transfected 293T cell line by TCF19 monoclonal antibody. Lane 1: TCF19 transfected lysate (37.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (TCF19 monoclonal antibody Western Blot analysis of TCF19 expression in Hela NE.)
Titration Curve (Titration curve analysis of LAG-3 mAbs to detect recombinant LAG-3 in ELISA with AAA11016 antibodies at decreasing concentrations.)
FCM (Flow Cytometry) (Flow cytometry analysis of LAG-3 over expressing HEK293 cell using LAG-3 antibody at 1 uh/mlBlue: Untransfected HEK293 cellsYellow: LAG-3 over expressing in HEK293 cells.)
IHC (Immunohistochemistry) (Immunohistochemistry of LAG-3 in human lymphoma tissue using LAG-3 Antibody and control mouse IgG at 5 ug/ml.)
IF (Immunofluorescence) (Immunofluorescence of LAG-3 in over human slpeen tissue using LAG-3 Antibody at 10 ug/mlRed: LAG3 Antibody [2G8] (AAA11016)Blue: DAPI staining)
ICC (Immunocytochemistry) (Immunocytochemistry of LAG-3 in over expressing HEK293 cells using LAG-3 antibody and control mouse IgG antibody at 1 ug/ml.)
Application Data (Figure A. StarBright Blue 810 conjugated Mouse anti Human CD4 and Alexa Fluor 647 conjugated Mouse IgG2a isotype control. Figure B. StarBright Blue 810 conjugated Mouse anti Human CD4 and Alexa Fluor 647 conjugated Mouse anti Human CD3. All experiments performed on red cell lysed human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Application Data (Figure A. StarBright Violet 570 conjugated Mouse anti Human CD4 and Alexa Fluor 488 conjugated Mouse IgG2a isotype control. Figure B. StarBright Violet 570 conjugated Mouse anti Human CD4 and Alexa Fluor 488 conjugated Mouse anti Human CD3. All experiments performed on red cell lysed human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Application Data (Figure A. StarBright Violet 710 conjugated Mouse anti Human CD4 and Pacific Blue conjugated Mouse IgG2a isotype control . Figure B. StarBright Violet 710 conjugated Mouse anti Human CD4 and Pacific Blue conjugated Mouse anti Human CD3 . All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Application Data (Figure A. StarBright Blue 580 conjugated Mouse anti Human CD4 and Alexa Fluor 700 conjugated Mouse IgG2a isotype control . Figure B. StarBright Blue 580 conjugated Mouse anti Human CD4 and Alexa Fluor 700 conjugated Mouse anti Human CD3 . All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Application Data (Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD4 and PE conjugated Mouse IgG2a isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD4 and PE conjugated Mouse anti Human CD3 . All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Application Data (Figure A. StarBright Violet 790 conjugated Mouse anti Human CD4 and FITC conjugated Mouse IgG2a isotype control . Figure B. StarBright Violet 790 conjugated Mouse anti Human CD4 and FITC conjugated Mouse anti Human CD3 . All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
WB (Western Blot) (NEK11 monoclonal antibody. Western Blot analysis of NEK11 expression in A-431.)
WB (Western Blot) (NEK11 monoclonal antibody, Western Blot analysis of NEK11 expression in HL-60.)
Application Data (Detection limit for recombinant GST tagged NEK11 is 0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NEK11 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NEK11 on formalin-fixed paraffin-embedded human endometrium tissue. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of NEK11 expression in transfected 293T cell line by NEK11 monoclonal antibody. Lane 1: NEK11 transfected lysate (55.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (79.13kD).)
FCM (Flow Cytometry) (Flow cytometric analysis of A431 cells with PAK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining PAK2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PAK2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PAK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PAK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PAK2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PAK2 on mouse thymus tissue (1) and MCF-7 cell (2) lysate using anti-PAK2 antibody at 1/500 dilution.)
WB (Western Blot) (Western blot analysis of SP110 over-expressed 293 cell line, cotransfected with SP110 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with SP110 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged SP110 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of SP110 transfected lysate using SP110 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SP110 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP110 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of SP110 expression in transfected 293T cell line by SP110 monoclonal antibody. Lane 1: SP110 transfected lysate (61.8kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SP110 monoclonal antibody. Western Blot analysis of SP110 expression in Jurkat.)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
IPR1 (SP110, Sp110 Nuclear Body Protein, Interferon-induced Protein 41/75, Speckled 110kD, Transcriptional Coactivator Sp110) (Biotin)
Gene Names
SP110; IPR1; VODI; IFI41; IFI75
Reactivity
Human
Applications
ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Purity
Purified by Protein A Affinity Chromatography.
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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