Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using Bcl-2 Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Bcl-2 Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using Bcl-2 Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using Bcl-2 Mouse mAb at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using Bcl-2 Mouse mAb at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil using Bcl-2 Mouse mAb at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human thyroid cancer using Bcl-2 Mouse mAb at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Bcl-2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

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Bcl-2, Polyclonal Antibody (Cat# AAA28424)

Full Name

Bcl-2 Mouse mAb

Gene Names

VEGFA; VPF; VEGF; MVCD1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purity

Affinity purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

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mTOR, Polyclonal Antibody (Cat# AAA28936)

Full Name

mTOR (phospho Ser2448) Polyclonal Antibody

Gene Names

MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

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Integrin alpha4, Polyclonal Antibody (Cat# AAA29192)

Full Name

Integrin alpha4 Polyclonal Antibody

Gene Names

ITGA4; IA4; CD49D

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining Ras in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Ras in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Ras in HCT116 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney muscle tissue using anti-Ras antibody. Counter stained with hematoxylin.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Ras antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-Ras antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Ras antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Ras antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Ras antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Ras on different cell lysates using anti-Ras antibody at 1/1000 dilution. Positive control: Lane 1: PC12 Lane 2: MCF-7 Lane 3: 293T Lane 4: Mouse brain Lane 5: Mouse intestine Lane 6: Rat brain)

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RAS, Polyclonal Antibody (Cat# AAA29960)

Full Name

RAS Antibody

Gene Names

NRAS; NS6; ALPS4; N-ras; NRAS1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Peptide affinity purified

Pricing

$360/0.05 mL $480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,925/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using VDAC2 antibody.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat heart using VDAC2 Rabbit pAb.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using VDAC2 Rabbit pAb.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using VDAC2 Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using VDAC2 Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using VDAC2 Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human brain using VDAC2 Rabbit pAb.)

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VDAC2, Polyclonal Antibody (Cat# AAA30728)

Full Name

VDAC2 Rabbit Polyclonal Antibody

Gene Names

VDAC2; POR

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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WB (Western Blot) (Figure 1. Western blot analysis of FOXK1 using anti-FOXK1 antibody.)

IHC (Immunohistochemistry)

IHC (Immunohistochemistry)

IF (Immunofluorescence)

IF (Immunofluorescence)

FCM (Flow Cytometry)

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FOXK1/MNF, Polyclonal Antibody (Cat# AAA19209)

Full Name

Anti-FOXK1/MNF Antibody

Gene Names

FOXK1; FOXK1L

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat lung using MAP2K4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Human colon cancer using MAP2K4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse kidney using MAP2K4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat ovary using MAP2K4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse liver using MAP2K4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of various lysates using MAP2K4 Polyclonal Antibody at 1:1000 dilution.)

WB (Western Blot) (Western blot analysis of A-549 using MAP2K4 Polyclonal Antibody at 1:1000 dilution.)

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MAP2K4, Polyclonal Antibody (Cat# AAA22281)

Full Name

MAP2K4 Polyclonal Antibody

Gene Names

MAP2K4; JNKK; MEK4; MKK4; SEK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$300/0.06 mL $415/0.12 mL $635/0.2 mL $1,225/2x0.2 mL $1,785/3x0.2 mL $2,310/4x0.2 mL $2,775/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

Application Data

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

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AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26882)

Full Name

AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (PE)

Gene Names

ACACA; ACC; ACAC; ACC1; ACCA; ACACAD

Reactivity

Mouse, Human

Applications

Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,115/0.1 mL $2,160/2x0.1 mL $3,150/3x0.1 mL $4,085/4x0.1 mL $4,790/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using VAPB antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using VAPB Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using VAPB Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney cancer using VAPB Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using VAPB Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using VAPB Antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using VAPB antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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VAPB, Polyclonal Antibody (Cat# AAA28162)

Full Name

VAPB Polyclonal Antibody

Gene Names

VAPB; ALS8; VAP-B; VAMP-B

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat brain using SCAF4 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse pancreas using SCAF4 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human placenta using SCAF4 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophagus using SCAF4 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of Rat thymus, using SCAF4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.)

WB (Western Blot) (Western blot analysis of extracts of HeLa cells, using SCAF4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.)

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SCAF4, Polyclonal Antibody (Cat# AAA28418)

Full Name

SCAF4 Rabbit pAb

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

$270/0.02 mL $470/0.1 mL $905/2x0.1 mL $1,315/3x0.1 mL $1,700/4x0.1 mL $1,870/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat brain using SCAF4 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse pancreas using SCAF4 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human placenta using SCAF4 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophagus using SCAF4 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of Rat thymus using SCAF4 Polyclonal Antibody at 1:1000 dilution.)

WB (Western Blot) (Western blot analysis of extracts of HeLa cells using SCAF4 Polyclonal Antibody at 1:1000 dilution.)

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SCAF4, Polyclonal Antibody (Cat# AAA22275)

Full Name

SCAF4 Polyclonal Antibody

Gene Names

SCAF4; SRA4; SFRS15

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

$295/0.06 mL $405/0.12 mL $615/0.2 mL $1,185/2x0.2 mL $1,725/3x0.2 mL $2,235/4x0.2 mL $2,730/5x0.2 mL

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WB (Western Blot) (WB Suggested Anti-GCLC Antibody Titration: 0.2-1 ug/mlPositive Control: Human Liver)

WB (Western Blot) (WB Suggested Anti-GCLC Antibody Titration: 0.2-1 ug/ml.A: - blocking peptide.B: + blocking peptide.)

WB (Western Blot) (Lanes:1: 40ug mouse heart lysate, 2: 40ug mouse heart lysate, 3: 40ug mouse heart lysate, 4: 40ug mouse heart lysate, 5: 40ug mouse heart lysatePrimary Antibody Dilution:1:1000Secondary Antibody:Anti-rabbit HRPSecondary Antibody Dilution:1:10000Gene Name:GCLCSubmitted by:Anonymous)

WB (Western Blot) (Host: RabbitTarget Name: GCLCSample Tissue: Mouse HeartAntibody Dilution: 1ug/ml)

WB (Western Blot) (Host: MouseTarget Name: GCLCSample Tissue: Mouse HeartAntibody Dilution: 1ug/ml)

IHC (Immunohistochemistry) (GCLC antibody - N-terminal region Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue Observed Staining: Cytoplasm and membrane of bronchial epithelial tissuePrimary Antibody Concentration: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec)

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GCLC, Polyclonal Antibody (Cat# AAA23555)

Full Name

GCLC antibody - N-terminal region

Gene Names

GCLC; GCL; GCS; GLCL; GLCLC

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of 293T cells using anti-MYNN antibody (AAA19970).Overlay histogram showing 293T cells stained with AAA19970 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MYNN Antibody (AAA19970, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of MYNN using anti-MYNN antibody (AAA19970) and anti-Beta Tubulin antibody (M01857-3).MYNN was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYNN Antibody (AAA19970) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYNN Antibody (AAA19970) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYNN Antibody (AAA19970) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Caco-2 whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human Hela whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYNN antigen affinity purified polyclonal antibody (#AAA19970) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MYNN at approximately 69 kDa. The expected band size for MYNN is at 69 kDa.)

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MYNN, Polyclonal Antibody (Cat# AAA19970)

Full Name

Anti-MYNN Antibody Picoband

Gene Names

MYNN; OSZF; SBBIZ1; ZBTB31; ZNF902

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat kidney using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of C6 cells, using Acetyl-Histone H4-K5/K8/K12/K16 antibody at 1:520 dilution.C6 cells were treated by TSA (1 uM) at 37 degree C for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

WB (Western Blot) (Western blot analysis of extracts of NIH/3T3 cells, using Acetyl-Histone H4-K5/K8/K12/K16 antibody at 1:520 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37 degree C for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)

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Acetyl-Histone H4-K5/K8/K12/K16, Polyclonal Antibody (Cat# AAA28423)

Full Name

Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb

Gene Names

FANCD2; FA4; FAD; FACD; FAD2; FA-D2; FANCD

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

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CD48, Polyclonal Antibody (Cat# AAA29191)

Full Name

CD48 Polyclonal Antibody

Gene Names

CD48; BCM1; BLAST; hCD48; mCD48; BLAST1; SLAMF2; MEM-102

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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WB (Western Blot) (WB Suggested Anti-LYN Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Hela cell lysateLYN is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells)

WB (Western Blot) (Host: RabbitTarget Name: LYNSample Type: Human Fetal LungAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: LYNSample Type: Human Fetal HeartAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: LYNSample Type: Human Fetal BrainAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: LYNSample Tissue: Mouse BrainAntibody Dilution: 1ug/ml)

WB (Western Blot) (Host: MouseTarget Name: LYNSample Tissue: Mouse BrainAntibody Dilution: 1ug/ml)

IHC (Immunohistochemistry) (Rabbit Anti-LYN AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Appendix (Colon) TissueObserved Staining: Plasma membranePrimary Antibody Concentration: 1:600Other Working Concentrations: N/ASecondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)

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LYN, Polyclonal Antibody (Cat# AAA23560)

Full Name

LYN antibody - N-terminal region

Gene Names

LYN; JTK8; p53Lyn; p56Lyn

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry Analysis: Representative lot data. (Fig. 1 and 2) Paraffin-embedded mouse and human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears as cytoplasmic. (Fig. 3 and 4) Paraffin-embedded mouse and mouse olfactory lobe and cerebellum brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a Chicken IgY Antibody 1:100 dilution of Cat. No. AB15894, anti-Tbr2. Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity seen here is mostly nuclear.)

IHC (Immunohistochemistry) (Paraffin-embedded mouse cerebellum was prepared using epitope retrieval in citrate buffer, pH 6.0. Staining was performed using 1:100 dilution. Immuno-reactivity is mostly nuclear.)

IHC (Immunohistochemistry) (Paraffin-embedded mouse olfactory lobe was prepared using epitope retrieval in citrate buffer, pH 6.0. Staining was performed using a 1:100 dilution. Reactivity is mostly nuclear.)

IS (Immunostaining) (Paraffin-embedded human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Staining pattern appears as cytoplasmic.)

IS (Immunostaining) (Paraffin-embedded mouse brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Staining pattern appears as cytoplasmic.)

WB (Western Blot) (Western Blot Analysis: Representative lot data. E13-14 mouse brain lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with (1:10,000) dilution. Proteins were visualized using a Rabbit Anti-Chicken conjugated to HRP and a chemi-luminescence detection system. Arrow indicates Tbr2 (~73 kD).)

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EOMES, Polyclonal Antibody (Cat# AAA26885)

Full Name

EOMES (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)

Gene Names

Eomes; Tbr2; TBR-2; C77258

Reactivity

Mouse, Human, Rat

Applications

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by affinity chromatography.

Pricing

$1,285/0.1 mL $2,495/2x0.1 mL $3,635/3x0.1 mL $4,715/4x0.1 mL $5,565/5x0.1 mL

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

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CD1D, Polyclonal Antibody (Cat# AAA29189)

Full Name

CD1D Polyclonal Antibody

Gene Names

CD1D; R3; CD1A

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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ChIP (Chromatin Immunoprecipitation) (Chromatin immunoprecipitation of extracts of 293T cell line, using HDAC3 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)

IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug HDAC3 antibody. Western blot was performed from the immunoprecipitate using HDAC3 antibody at a dilition of 1:1000.)

IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using HDAC3 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using HDAC3 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophageal cancer using HDAC3 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophagus using HDAC3 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using HDAC3 Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using HDAC3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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HDAC3, Polyclonal Antibody (Cat# AAA10758)

Full Name

HDAC3 Polyclonal Antibody

Gene Names

HDAC3; HD3; RPD3; RPD3-2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), CHIP

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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WB (Western Blot) (PEPP-2 polyclonal antibody. Western Blot analysis of PEPP-2 expression in K-562.)

IF (Immunofluorescence) (Immunofluorescence of purified antibody to PEPP-2 on HeLa cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (Western Blot analysis of RHOXF2 expression in transfected 293T cell line by RHOXF2 polyclonal antibody. Lane 1: PEPP-2 transfected lysate (31.79kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (RHOXF2 polyclonal antibody. Western Blot analysis of RHOXF2 expression in NIH/3T3.)

WB (Western Blot) (RHOXF2 polyclonal antibody. Western Blot analysis of RHOXF2 expression in Raw 264.7.)

WB (Western Blot) (RHOXF2 polyclonal antibody. Western Blot analysis of RHOXF2 expression in PC-12.)

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RHOXF2, Polyclonal Antibody (Cat# AAA24070)

Full Name

RHOXF2 (Rhox Homeobox Family Member 2, Paired-like Homeobox Protein PEPP-2, Testis Homeobox Gene 1, PEPP2, THG1, CT107)

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunofluorescence (IF)

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$770/0.05 mg $1,490/2x0.05 mg $2,170/3x0.05 mg $2,810/4x0.05 mg $3,235/5x0.05 mg

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FCM (Flow Cytometry) (IDH1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot) (Western blot analysis of IDH1 Antibody (Center) in HepG2 cell line and mouse liver tissue lysates (35ug/lane). IDH1 (arrow) was detected using the purified Pab.)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDH1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

IF (Immunofluorescence) (Confocal immunofluorescent analysis of IDH1 Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

WB (Western Blot) (Western blot analysis of lysates from HepG2, MCF-7 cell line, human liver and rat liver tissue lysate(from left to right), using IDH1 Antibody (Center). AAA28676 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded H. prostate section using IDH1 Antibody (Center). AAA28676 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

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IDH1, Polyclonal Antibody (Cat# AAA28676)

Full Name

IDH1 Antibody (Center)

Gene Names

IDH1; IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26

Reactivity

Human, mouse (Predicted Reactivity: Bovine, Rat)

Applications

ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$250/0.08 mL $480/0.4 mL $925/2x0.4 mL $1,345/3x0.4 mL $1,740/4x0.4 mL $1,975/5x0.4 mL

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IHC (Immunohistochemistry) (Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

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JAK2, Polyclonal Antibody (Cat# AAA28932)

Full Name

JAK2 (phospho Tyr1007) Polyclonal Antibody

Gene Names

JAK2; JTK10; THCYT3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse liver using SFN Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophagus using SFN Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney using SFN Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human liver injury using SFN Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using SFN Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using SFN Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat liver using SFN Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using SFN Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SFN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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SFN, Polyclonal Antibody (Cat# AAA10757)

Full Name

SFN Polyclonal Antibody

Gene Names

SFN; YWHAS

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using HIBADH Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using HIBADH Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using HIBADH Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human esophageal using HIBADH Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human lung cancer using HIBADH Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using HIBADH Polyclonal Antibody at 1:3000 dilution.)

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HIBADH, Polyclonal Antibody (Cat# AAA22277)

Full Name

HIBADH Polyclonal Antibody

Gene Names

HIBADH; NS5ATP1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$295/0.06 mL $405/0.12 mL $615/0.2 mL $1,185/2x0.2 mL $1,725/3x0.2 mL $2,235/4x0.2 mL $2,730/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of rat bronchus using Cytokeratin 7 (Cytokeratin 7 (KRT7)) antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of mouse bronchus using Cytokeratin 7 (Cytokeratin 7 (KRT7)) antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of human lung cancer using Cytokeratin 7 (Cytokeratin 7 (KRT7)) antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using Cytokeratin 7 (Cytokeratin 7 (KRT7)) antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of Rat lung, using Cytokeratin 7 (KRT7) antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

WB (Western Blot) (Western blot analysis of extracts of HepG2 cells, using Cytokeratin 7 (KRT7) antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.)

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Cytokeratin 7 (KRT7), Polyclonal Antibody (Cat# AAA28419)

Full Name

Cytokeratin 7 (KRT7) Rabbit pAb

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purity

Affinity purification

Pricing

$270/0.02 mL $470/0.1 mL $905/2x0.1 mL $1,315/3x0.1 mL $1,700/4x0.1 mL $1,870/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using PDK4 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using PDK4 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using PDK4 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using PDK4 Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human placenta using PDK4 Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using PDK4 Rabbit pAb.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PDK4 antibody.)

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PDK4, Polyclonal Antibody (Cat# AAA30723)

Full Name

PDK4 Rabbit Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HCT116 cells using anti-SRPRA antibody (AAA19972).Overlay histogram showing HCT116 cells stained with AAA19972 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SRPRA Antibody (AAA19972, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 5. IF analysis of SRPRA using anti-SRPRA antibody (AAA19972).SRPRA was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-SRPRA Antibody (AAA19972) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 4. IF analysis of SRPRA using anti-SRPRA antibody (AAA19972).SRPRA was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SRPRA Antibody (AAA19972) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SRPRA Antibody (AAA19972) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human HEL whole cell lysates,Lane 4: rat liver tissue lysates,Lane 5: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRPRA antigen affinity purified polyclonal antibody (#AAA19972) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SRPRA at approximately 70 kDa. The expected band size for SRPRA is at 70 kDa.)

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SRPRA, Polyclonal Antibody (Cat# AAA19972)

Full Name

Anti-SRPRA Antibody Picoband

Gene Names

SRPR; DP; Sralpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer using CEACAM5 antibody at dilution of 100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Human urothelial carcinoma using CEACAM5 antibody at dilution of 100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human hepatocellular carcinoma using CEACAM5 antibody at dilution of 100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human colon carcinoma using CEACAM5 antibody at dilution of 100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human appendix using CEACAM5 antibody at dilution of 100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human tonsil (negative control sample) using CEACAM5 antibody at dilution of 100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of MCF7 cells, using CEACAM5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.)

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CEACAM5, Polyclonal Antibody (Cat# AAA28422)

Full Name

CEACAM5 Mouse mAb

Gene Names

SNRK2.3; SNRK2-3; SRK2I; SUCROSE NONFERMENTING 1 (SNF1)-RELATED PROTEIN KINASE 2-3; sucrose nonfermenting 1(SNF1)-related protein kinase 2.3

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Purity

Affinity purification

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

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CD2, Polyclonal Antibody (Cat# AAA29190)

Full Name

CD2 Polyclonal Antibody

Gene Names

CD2; T11; SRBC; LFA-2

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining MGMT in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining MGMT in SK-BR-3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining MGMT in A172 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-MGMT antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MGMT antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of MGMT on different cell lysates using anti- MGMT antibody at 1/1000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: Daudi Lane 4: Raji Lane 5: SK-BR-3 Lane 6: SKOV3 Lane 7: Human liver Lane 8: Human kidney Lane 9: Human thymus Lane 10: Human placentae)

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MGMT, Polyclonal Antibody (Cat# AAA29958)

Full Name

MGMT Antibody

Reactivity

Human

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Peptide affinity purified

Pricing

$360/0.05 mL $480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,925/5x0.1 mL

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WB (Western Blot) (WB Suggested Anti-GSK3B Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 721_B cell lysateGSK3B is supported by BioGPS gene expression data to be expressed in 721_B)

WB (Western Blot) (Host: RabbitTarget Name: GSK3BSample Type: Human Fetal HeartAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: GSK3BSample Type: HelaAntibody Dilution: 1.0ug/mlGSK3B is supported by BioGPS gene expression data to be expressed in HeLa)

WB (Western Blot) (Host: RabbitTarget Name: GSK3BSample Tissue: Mouse TestisAntibody Dilution: 1ug/ml)

WB (Western Blot) (Host: MouseTarget Name: GSK3BSample Tissue: Mouse TestisAntibody Dilution: 1ug/ml)

IHC (Immunohistochemistry) (Rabbit Anti-GSK3B AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Testis TissueObserved Staining: Cytoplasm, Nucleus, Plasma membranePrimary Antibody Concentration: 1:100Other Working Concentrations: 1:600Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)

IHC (Immunohistochemistry) (Sample Type: Human brainBrain, cortex)

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GSK3B, Polyclonal Antibody (Cat# AAA23559)

Full Name

GSK3B antibody - C-terminal region

Reactivity

Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining HSP70 in SKBR-3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining HSP70 in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining HSP70 in SHG-44 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-HSP70 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-HSP70 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-HSP70 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-HSP70 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of HSP70 on different cell lysates using anti-HSP70 antibody at 1/500 dilution. Positive control: Lane 1: A549 Lane 2: MCF-7 Lane 3: HCT116)

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HSP70, Polyclonal Antibody (Cat# AAA29967)

Full Name

HSP70 Antibody

Gene Names

HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Purity

Peptide affinity purified

Pricing

$360/0.05 mL $480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,925/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human lymphonodus using PAK1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using PAK1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat pancreas using PAK1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat spleen using PAK1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using PAK1 Antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of mouse kidney, using PAK1 antibody at 1:700 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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PAK1, Polyclonal Antibody (Cat# AAA10768)

Full Name

PAK1 Polyclonal Antibody

Gene Names

PAK1; PAKalpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of RAW264.7 cells using anti-CD326/Epcam antibody (AAA19728).Overlay histogram showing RAW264.7 cells stained with AAA19728 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD326/Epcam Antibody (AAA19728, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of CD326/Epcam using anti-CD326/Epcam antibody (AAA19728).CD326/Epcam was detected in a paraffin-embedded section of rat colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-CD326/Epcam Antibody (AAA19728) overnight at 4 degree C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 4. IF analysis of CD326/Epcam using anti-CD326/Epcam antibody (AAA19728).CD326/Epcam was detected in a paraffin-embedded section of mouse colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-CD326/Epcam Antibody (AAA19728) overnight at 4 degree C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of CD326/Epcam using anti-CD326/Epcam antibody (AAA19728).CD326/Epcam was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-CD326/Epcam Antibody (AAA19728) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-CD326/Epcam Antibody (AAA19728) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD326/Epcam antigen affinity purified polyclonal antibody (#AAA19728) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (40 kDa.)

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CD326/Epcam, Polyclonal Antibody (Cat# AAA19728)

Full Name

Anti-CD326/Epcam Antibody Picoband

Gene Names

Epcam; EGP; Ly74; gp40; CD326; EGP-2; TROP1; Egp314; Ep-CAM; EpCAM1; Tacsd1; GA733-2; Tacstd1

Reactivity

Mouse, Rat

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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WB (Western Blot) (Western Blot; Sample: Human Lung lysatePrimary Ab: 0.01ug/ml Rabbit Anti-Human CK19 AntibodySecond Ab: 0.2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

WB (Western Blot) (Western Blot; Samples: Lane1: HepG2 cell lysate; Lane2: MCF7 cell lysate; Lane3: Human Serum; Primary Ab: 0.1ug/ml Rabbit Anti-Human CK19 Antibody Second Ab: 0.2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry-Paraffin) (DAB staining on IHC-P;Sample: Porcine Liver TissuePrimary Ab: 10ug/ml Rabbit Anti-Human CK19 AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry-Paraffin) (DAB staining on IHC-P;Sample: Porcine Pancreas TissuePrimary Ab: 10ug/ml Rabbit Anti-Human CK19 AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry-Paraffin) (DAB staining on IHC-P;Sample: Porcine Small intestine TissuePrimary Ab: 10ug/ml Rabbit Anti-Human CK19 AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry-Paraffin) (DAB staining on IHC-P;Sample: Porcine Uterus TissuePrimary Ab: 10ug/ml Rabbit Anti-Human CK19 AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

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Cytokeratin 19 (CK19), Polyclonal Antibody (Cat# AAA21264)

Full Name

Polyclonal Antibody to Cytokeratin 19 (CK19)

Gene Names

KRT19; K19; CK19; K1CS

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$220/0.1 mL $275/0.2 mL $425/0.5 mL $510/1 mL $1,355/5 mL $2,630/2x5 mL $3,835/3x5 mL $4,975/4x5 mL $6,080/5x5 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of PC-3 cells using PDIA4 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using PDIA4 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using PDIA4 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using PDIA4 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using PDIA4 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using PDIA4 Polyclonal Antibody at 1:1000 dilution.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using PDIA4 Polyclonal Antibody at 1:1000 dilution.)

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PDIA4, Polyclonal Antibody (Cat# AAA22288)

Full Name

PDIA4 Polyclonal Antibody

Gene Names

PDIA4; ERP70; ERP72; ERp-72

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$300/0.06 mL $415/0.12 mL $635/0.2 mL $1,225/2x0.2 mL $1,785/3x0.2 mL $2,310/4x0.2 mL $2,775/5x0.2 mL

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

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CD84, Polyclonal Antibody (Cat# AAA29198)

Full Name

CD84 Polyclonal Antibody

Gene Names

CD84; LY9B; hCD84; mCD84; SLAMF5

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.