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Secondary Antibodies
At AAA Biotech, we provide a vast collection of high-quality, purified, conjugated and unconjugated secondary antibodies to use in various applications, such as Western Blotting, Flow Cytometry, Cell Imaging, or CUT & Tag.
Our secondary antibodies are available with a wide range of labels—such as HRP, AP, FITC, and biotin—to match your detection system and enhance signal sensitivity. Each antibody is rigorously tested to ensure high specificity and minimal cross-reactivity, delivering reliable performance across multiple species and assay types.
Whether you're conducting qualitative imaging or quantitative analysis, AAA Biotech has the right secondary antibody to support your research with consistency and precision. Our entire catalog of secondary antibodies is available for browsing through our main website.
Viewing 1-17 of 17 product results
anti-human IgG, Monoclonal Secondary Antibody (Cat# AAA13530)
Immunohistochemistry (IHC) (1:500-1,000)
Immunoprecipitation ( IP ) (1, 5,000- 20, 000)
Immunofluorescence (IF) ( 1,5000-20, 000)
ELISA (1: 5,000 - 20, 000)
Mouse Anti-Monkey IgG, Monoclonal Secondary Antibody (Cat# AAA14894)
Goat F(ab) Anti-Human IgG (gamma chain specific), Secondary Antibody (Cat# AAA14905)
Rat Anti-Mouse Lambda (lambda light chain specific), Monoclonal Secondary Antibody (Cat# AAA14893)
Goat anti Rabbit IgG, Polyclonal Secondary Antibody (Cat# AAA14909)
Goat Anti-Human IgG (gamma chain specific), Polyclonal Secondary Antibody (Cat# AAA14904)
Goat Anti-Rat IgG (gamma chain specific), Polyclonal Secondary Antibody (Cat# AAA14908)
Goat F(ab')2 Anti-Mouse kappa (kappa chain specific), Polyclonal Secondary Antibody (Cat# AAA14899)
anti-Rabbit IgG+HRP, Secondary Antibody (Cat# AAA13854)
Affinity purified and conjugated with horseradish peroxidase (HRP)
Ig, Polyclonal Secondary Antibody (Cat# AAA14101)
Alkaline phosphatase, Polyclonal Secondary Antibody (Cat# AAA14876)
Goat F(ab')2 Anti-Human IgM (u chain specific), Polyclonal Secondary Antibody (Cat# AAA14901)
IgG Fc fragment, Polyclonal Secondary Antibody (Cat# AAA15949)
Goat anti Human IgG Fc, Secondary Antibody (Cat# AAA14316)
Goat F(ab')2 Anti-Human IgG (gamma chain specific), Polyclonal Secondary Antibody (Cat# AAA14907)
Goat Anti-Human IgG, Monkey ads, Secondary Antibody (Cat# AAA14913)
IgG (H+L), Secondary Antibody (Cat# AAA26993)
What Are Secondary Antibodies?
Secondary antibodies are immunoglobulins that bind to primary antibodies, which are directly bound to the target antigen. They are conjugated with either an enzyme, fluorophore, or biotin, and they are almost always only targeting the constant region (Fc) of the primary antibody. As a result, this amplifies the signal in various assays and helps researchers detect, quantify, and visualize specific antigens in a complex biological sample.
Key Applications of Secondary Antibodies
- Western blotting for protein detection and quantification.
- ELISA (Enzyme-Linked Immunosorbent Assay) for antigen-antibody interaction studies and quantification.
- Immunohistochemistry (IHC) and immunofluorescence imaging for tissue and cell analysis.
- Flow cytometry for cell surface and intracellular marker detection.
- Immunoprecipitation (IP) and chromatin immunoprecipitation (ChIP) assays.
- Signal amplification in various immunoassays.
- Multiplex assays using fluorophore-conjugated secondary antibodies.
Advantages of Using Secondary Antibodies
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High sensitivity
Multiple secondary antibodies can bind to a single primary antibody and amplify the signal. This is especially useful for detecting antigens even if they are in low concentrations.
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Increased flexibility in assay design
Secondary antibodies come pre-conjugated with labels, such as enzymes, fluorophores, biotin, and more. This provides researchers with flexibility to use in multiple applications. They also support multiplexing for the simultaneous detection of multiple targets.
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Cost-effective
Since the Fc domain remains constant within the same animal class, only one type of secondary antibody is needed to bind many types of primary antibodies. This reduces the cost of labeling multiple primary antibodies.
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Better signal-to-noise ratio
Secondary antibodies minimize the background noise. As a result, they provide clearer and more accurate results.
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Versatility
A single species- and isotype-specific secondary antibody can be used with any compatible primary antibody. This can help streamline reagent inventory and reduce cost and preparation time.
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Easier detection
Unlike primary antibodies, secondary antibodies’ main characteristic is that they will almost always be partnered with a component that can be used for detection. This can make detection and visualization easy.
Why Buy Secondary Antibodies from AAA Biotech?
- Highly Validated: Most of our secondary antibodies are thoroughly tested to ensure they work reliably across different experiments.
- Versatile Applications: Our secondary antibodies can be used in a wide range of research techniques, including (but not limited to) immunocytochemistry (ICC), ELISA, immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FC), immunoprecipitation (IP), and Western blotting (WB).
- Support for Rare Species: We offer secondary antibodies specifically designed for rare or less common species—something many other suppliers don’t provide.
- Affordable Prices: Our secondary antibodies are available at competitive prices to support all research budgets.
- Quick and Convenient Ordering: Simple online ordering process with responsive customer support.
- Privacy: We respect your privacy and ensure your information is protected at every step of the ordering process, and in all other communication with our team members.
FAQ
1. Can I reuse a secondary antibody?
Not recommended for critical experiments, as reusing may compromise sensitivity and specificity.
2. What is the difference between primary and secondary antibodies?
Primary antibodies bind directly to the antigen. Secondary antibodies bind to the primary and are usually labeled for detection.
3. How long do secondary antibodies last?
When stored properly (usually at –20°C or 4°C, depending on formulation), they can last for years. Always refer to the product datasheet.
4. What happens if you use too much secondary antibody?
Excess can increase background noise, leading to a poor signal-to-noise ratio. Titrate to find the optimal concentration.
5. Can secondary antibodies be monoclonal?
Yes. While many are polyclonal, monoclonal secondary antibodies are available for consistent batch-to-batch performance.
6. How to find secondary antibodies?
Use our catalog filters to search by host species, conjugate type, target species, and application.
7. How to store secondary antibodies?
Store at recommended temperatures away from light. Avoid repeated freeze-thaw cycles.