Loading...

Skip to main content
Call us at +1-800-604-9114 for more information about our products or contact us online

Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.


Viewing 1-50 of 266 product results


RIPK2, ELISA Kit (Cat# AAA30733)

Full Name
Phospho-RIPK2 (Ser176) Colorimetric Cell-Based ELISA Kit
Gene Names
RIPK2; CCK; RICK; RIP2; CARD3; GIG30; CARDIAK
Reactivity
Human, Mouse
Pricing
product-image-AAA28936_IHC7.jpg IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

mTOR, Polyclonal Antibody (Cat# AAA28936)

Full Name
mTOR (phospho Ser2448) Polyclonal Antibody
Gene Names
MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
Pricing
product-image-AAA14088_APP.gif Application Data

STAT3 (pY705), Antibody (Cat# AAA14088)

Full Name
Rabbit anti Phospho- STAT3 (pTyr 705)
Gene Names
STAT3; APRF; HIES; FLJ20882; MGC16063
Reactivity
Human, Rat, Mouse, Canine, Bovine, Chicken
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
Pricing
product-image-AAA28932_IHC7.jpg IHC (Immunohistochemistry) (Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

JAK2, Polyclonal Antibody (Cat# AAA28932)

Full Name
JAK2 (phospho Tyr1007) Polyclonal Antibody
Gene Names
JAK2; JTK10; THCYT3
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Pricing
product-image-AAA24080_WB7.jpg WB (Western Blot) (Western blot analysis of ENO3 over-expressed 293 cell line, cotransfected with ENO3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ENO3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

ENO3, Monoclonal Antibody (Cat# AAA24080)

Full Name
ENO3 (Enolase 3, 2-phospho-D-glycerate Hydro-lyase, beta-Enolase, Muscle-specific Enolase, MSE, Skeletal Muscle Enolase)
Gene Names
ENO3; MSE; GSD13
Reactivity
Human
Applications
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Pricing
product-image-AAA27919_WB.png WB (Western Blot) (Western blot analysis of c-Abl phosphorylation expression in Insulin treated K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

c-Abl, Antibody (Cat# AAA27919)

Full Name
Phospho-c-Abl (Tyr245) Antibody
Gene Names
ABL1; ABL; JTK7; p150; c-ABL; v-abl; CHDSKM; c-ABL1; bcr/abl
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Purity
Immunogen affinity purified
Pricing
product-image-AAA28943_IHC7.jpg IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 WB 1:500-2000, IHC 1:50-300 IHC 1:50-300)

Tau, Polyclonal Antibody (Cat# AAA28943)

Full Name
Tau (phospho Ser396) Polyclonal Antibody
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
Pricing
product-image-AAA28940_IHC7.jpg IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications.)

Stat1, Polyclonal Antibody (Cat# AAA28940)

Full Name
Stat1 (phospho Tyr701) Polyclonal Antibody
Gene Names
STAT1; CANDF7; ISGF-3; STAT91
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunoprecipitation (IP)
Pricing
product-image-AAA14094_APP.gif Application Data

FAK (pY576), Antibody (Cat# AAA14094)

Full Name
Rabbit anti Phospho-FAK (pTyr 576)
Reactivity
Human; Rat; Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
Pricing
product-image-AAA30744_WB6.png WB (Western Blot) (Western Blot analysis of various cells using Phospho-Cdk2/Cdc2 (T160) Polyclonal Antibody diluted at 1:500)

Cdk2/Cdc2, Polyclonal Antibody (Cat# AAA30744)

Full Name
Cdk2/Cdc2 (Phospho-Thr160) Rabbit Polyclonal Antibody
Gene Names
CDK2; p33(CDK2)
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
product-image-AAA30745_IHC11.png IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,NF?B-p65 (phospho Ser529) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

NFkappaB-p65, Polyclonal Antibody (Cat# AAA30745)

Full Name
NFkappaB-p65 (Phospho-Ser529) Rabbit Polyclonal Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse, Rat, Monkey
Applications
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA)
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
product-image-AAA28956_IHC6.jpg IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

MOR-1, Polyclonal Antibody (Cat# AAA28956)

Full Name
MOR-1 (phospho Ser375) Polyclonal Antibody
Gene Names
OPRM1; MOP; MOR; LMOR; MOR1; OPRM; M-OR-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Pricing
product-image-AAA28958_IHC7.jpg IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

ERK 1/2, Polyclonal Antibody (Cat# AAA28958)

Full Name
ERK 1/2 (phospho Thr202/Y204) Polyclonal Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
Pricing
product-image-AAA17961_IHC.jpg IHC (Immunohistochemistry) (P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Histone H2A (Phospho-Thr121) antibody.)

Histone H2A, Antibody (Cat# AAA17961)

Full Name
Histone H2A (Phospho-Thr121) Antibody
Gene Names
HIST1H2AI; H2A/c; H2AFC
Reactivity
Human (Identities = 100%, Positives = 100%)
Mouse (Identities = 100%, Positives = 100%)
Rat (Identities = 100%, Positives = 100%)
Applications
Immunohistochemistry (IHC), ELISA (EIA)
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA31288_APP7.jpg Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

PFKFB3, Polyclonal Antibody (Cat# AAA31288)

Full Name
Phospho-PFKFB3 (Ser461) Antibody
Gene Names
PFKFB3; PFK2; IPFK2
Reactivity
Human, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA16702_SC.jpg Standard Curve (Sample)

Phospho Spleen tyosine kinase, ELISA Kit (Cat# AAA16702)

Full Name
Mouse Phospho Spleen tyosine kinase ELISA Kit
Reactivity
Mouse
Pricing
product-image-AAA31294_IHC8.jpg IHC (Immunohistochemistry) (At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

YB1, Polyclonal Antibody (Cat# AAA31294)

Full Name
Phospho-YB1 (Ser102) Antibody
Gene Names
YBX1; YB1; BP-8; CSDB; DBPB; YB-1; CSDA2; NSEP1; NSEP-1; MDR-NF1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry (IHC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31290_IHC6.jpg IHC (Immunohistchemistry) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

PKC theta, Polyclonal Antibody (Cat# AAA31290)

Full Name
Phospho-PKC theta (Ser695) Antibody
Gene Names
PRKCQ; PRKCT; nPKC-theta
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry (IHC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31309_APP7.jpg Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

Lamin A/C, Polyclonal Antibody (Cat# AAA31309)

Full Name
Phospho-Lamin A/C (Ser390) Antibody
Gene Names
LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31307_APP6.jpg Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

EPRS, Polyclonal Antibody (Cat# AAA31307)

Full Name
Phospho-EPRS (Ser886) Antibody
Gene Names
EPRS; EARS; PARS; QARS; QPRS; PIG32; GLUPRORS
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA29772_ICC6.jpg ICC (Immunocytochemistry) (ICC staining Phospho-GSK3 (alpha+beta) (Y216+Y279) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

GSK3 (alpha+beta), Monoclonal Antibody (Cat# AAA29772)

Full Name
GSK3 (alpha+beta) (Phospho-Y216+Y279) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Purity
ProA affinity purified
Pricing
product-image-AAA31312_APP10.jpg Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

Bub1, Polyclonal Antibody (Cat# AAA31312)

Full Name
Phospho-Bub1 (Ser459) Antibody
Gene Names
BUB1; BUB1A; BUB1L; hBUB1
Reactivity
Human, Rat
Applications
Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31310_APP9.jpg Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

SNAIL, Polyclonal Antibody (Cat# AAA31310)

Full Name
Phospho-SNAIL (Ser11) Antibody
Gene Names
SNAI1; SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31314_IHC6.jpg IHC (Immunohistchemistry) (At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

ARRB1, Polyclonal Antibody (Cat# AAA31314)

Full Name
Phospho-ARRB1 (Ser412) Antibody
Gene Names
ARRB1; ARB1; ARR1
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31306_APP13.jpg Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

TOP2A, Polyclonal Antibody (Cat# AAA31306)

Full Name
Phospho-TOP2A (Thr1343) Antibody
Gene Names
TOP2A; TOP2; TP2A
Reactivity
Human, Mouse
Applications
Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA29769_ICC10.jpg ICC (Immunocytochemistry) (ICC staining Phospho-Rb (S807) in CRC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

RB, Monoclonal Antibody (Cat# AAA29769)

Full Name
Rb (Phospho-S807) Antibody
Gene Names
RB1; RB; pRb; OSRC; pp110; p105-Rb
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Purity
ProA affinity purified
Pricing
product-image-AAA31327_IHC7.jpg IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Cytokeratin 18, Polyclonal Antibody (Cat# AAA31327)

Full Name
Phospho-Cytokeratin 18 (Ser51) Antibody
Gene Names
KRT18; K18; CYK18
Reactivity
Human, Mouse
Applications
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA28260_IF7.jpg IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using Phospho-JNK1/2/3-T183/T183/T221 antibody.)

JNK1/2/3-T183/T183/T221, Monoclonal Antibody (Cat# AAA28260)

Full Name
Phospho-JNK1/2/3-T183/T183/T221 Monoclonal Antibody
Gene Names
MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Purity
Affinity Purification
Pricing
product-image-AAA31324_IHC18.jpg IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

AIRE, Polyclonal Antibody (Cat# AAA31324)

Full Name
Phospho-AIRE (Ser156) Antibody
Gene Names
AIRE; APS1; APSI; PGA1; AIRE1; APECED
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry (IHC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31351_IHC8.jpg IHC (Immunohistochemistry) (At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Histone H2B, Polyclonal Antibody (Cat# AAA31351)

Full Name
Phospho-Histone H2B (Tyr43) Antibody
Reactivity
Human, Mouse
Applications
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31343_IHC11.jpg IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

KSR2, Polyclonal Antibody (Cat# AAA31343)

Full Name
Phospho-KSR2 (Thr497) Antibody
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (91%), Xenopus (91%)
Applications
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31297_APP12.jpg Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

GSK3 beta, Polyclonal Antibody (Cat# AAA31297)

Full Name
Phospho-GSK3 beta (Thr390) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31303_APP8.jpg Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

CREB, Polyclonal Antibody (Cat# AAA31303)

Full Name
Phospho-CREB (Ser129) Antibody
Gene Names
CREB1; CREB
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31304_APP7.jpg Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

RPA32/RPA2, Polyclonal Antibody (Cat# AAA31304)

Full Name
Phospho-RPA32/RPA2 (Ser4/Ser8) Antibody
Gene Names
RPA2; REPA2; RPA32
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA17987_WB2.jpg WB (Western Blot) (Western blot analysis of extracts from COLO205 cells using Src (Ab-418) antibody and Src (phospho-Tyr418) antibody.)

Src, Antibody (Cat# AAA17987)

Full Name
Src (Phospho-Tyr418) Antibody
Gene Names
SRC; ASV; SRC1; THC6; c-SRC; p60-Src
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC)
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA31321_APP19.jpg Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

4E-BP1, Polyclonal Antibody (Cat# AAA31321)

Full Name
Phospho-4E-BP1 (Thr37/Thr46) Antibody
Gene Names
EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31315_IHC20.jpg IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

RSK1/2/3/4, Polyclonal Antibody (Cat# AAA31315)

Full Name
Phospho-RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232) Antibody
Gene Names
RPS6KA1; RSK; HU-1; RSK1; MAPKAPK1A
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31359_IHC7.jpg IHC (Immunohistochemistry) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

ULK1, Polyclonal Antibody (Cat# AAA31359)

Full Name
Phospho-ULK1 (Ser757)[Ser758] Antibody
Gene Names
ULK1; ATG1; ATG1A; UNC51; hATG1; Unc51.1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (88%)
Applications
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31361_IHC12.jpg IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

SRF, Polyclonal Antibody (Cat# AAA31361)

Full Name
Phospho-SRF (Ser103) Antibody
Gene Names
SRF; MCM1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Rabbit (89%), Dog (100%)
Applications
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31363_IHC11.jpg IHC (Immunohistochemistry) (At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

DUSP1/MKP1, Polyclonal Antibody (Cat# AAA31363)

Full Name
Phospho-DUSP1/MKP1 (Ser359) Antibody
Gene Names
DUSP1; HVH1; MKP1; CL100; MKP-1; PTPN10
Reactivity
Human, Mouse
Predicted Reactivity: Pig (100%), Zebrafish (92%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (92%)
Applications
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing

ENO3, Monoclonal Antibody (Cat# AAA24202)

Full Name
ENO3 (Enolase 3, 2-phospho-D-glycerate Hydro-lyase, beta-Enolase, Muscle-specific Enolase, MSE, Skeletal Muscle Enolase) (AP)
Gene Names
ENO3; MSE; GSD13
Reactivity
Human
Applications
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Purity
Purified by Protein A Affinity Chromatography.
Pricing
product-image-AAA31364_APP18.jpg Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

AKT1, Polyclonal Antibody (Cat# AAA31364)

Full Name
Phospho-AKT1 (Ser124) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (91%), Bovine (82%), Horse (100%), Dog (100%), Chicken (100%)
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31374_APP9.jpg Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

RSK1 p90, Polyclonal Antibody (Cat# AAA31374)

Full Name
Phospho-RSK1 p90 (Thr573) Antibody
Gene Names
RPS6KA1; RSK; HU-1; RSK1; MAPKAPK1A
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31385_APP8.jpg Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

TLK1, Polyclonal Antibody (Cat# AAA31385)

Full Name
Phospho-TLK1 (Ser741) Antibody
Gene Names
TLK1; PKU-beta
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31381_IHC12.jpg IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

NFAT2, Polyclonal Antibody (Cat# AAA31381)

Full Name
Phospho-NFAT2 (Ser294) Antibody
Gene Names
NFATC1; NFAT2; NFATc; NF-ATC
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (85%), Rabbit (100%)
Applications
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31391_APP6.jpg Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

HSF1, Polyclonal Antibody (Cat# AAA31391)

Full Name
Phospho-HSF1 (Ser307) Antibody
Gene Names
HSF1; HSTF1
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA24498_WB7.jpg WB (Western Blot) (Western blot analysis of ENO3 over-expressed 293 cell line, cotransfected with ENO3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ENO3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

ENO3, Monoclonal Antibody (Cat# AAA24498)

Full Name
ENO3 (Enolase 3, 2-phospho-D-glycerate Hydro-lyase, beta-Enolase, Muscle-specific Enolase, MSE, Skeletal Muscle Enolase) APC
Gene Names
ENO3; MSE; GSD13
Reactivity
Human
Applications
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Purity
Purified by Protein A Affinity Chromatography.
Pricing
product-image-AAA31410_APP6.jpg Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)

PKC theta, Polyclonal Antibody (Cat# AAA31410)

Full Name
Phospho-PKC theta (Tyr90) Antibody
Gene Names
PRKCQ; PRKCT; nPKC-theta
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (91%), Dog (91%)
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31152_WB6.jpg WB (Western Blot) (Western blot analysis of Phospho-JunD (Ser259) in lysates of K562 , using Phospho-JunD (Ser259) Antibody.)

JunD, Polyclonal Antibody (Cat# AAA31152)

Full Name
Phospho-JunD (Ser259) Antibody
Gene Names
JUND; AP-1
Reactivity
Human, Mouse, Rat
Predicted: Pig, Zebrafish, Bovine, Dog, Chicken
Applications
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31411_IHC17.jpg IHC (Immunohistochemistry) (At 1/200 staining Human tonsil tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

TBK1, Polyclonal Antibody (Cat# AAA31411)

Full Name
Phospho-TBK1 (Ser172) Antibody
Gene Names
TBK1; NAK; T2K
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing

What Are Phospho Antibodies?


Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.


Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.


What Are The Key Applications of Phospho Antibodies?


1. Western Blotting


One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.


2. Staining of Fixed Cells (Immunocytochemistry)


Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.


3. Specificity Tests Using Peptides


In order to make sure that the antibody is only binding to the right target:


  • Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).
  • If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.
  • A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.
  • Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation


As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.


Why Buy Phospho Antibodies Through Us?


  • The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:
  • Standard methods and proper controls in all tests to ensure high quality.
  • These antibodies are tested and validated in different cell types and species.
  • High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ


1. What Are Phospho-Specific Antibodies?


Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.


2. How to Detect Phosphorylated Proteins in a Western Blot?


To find out if a protein is phosphorylated using Western blot:


  • Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.
  • You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?


Here are some simple tips to help you pick the right antibody:


  • Know your target
  • Match your sample characteristics
  • Confirm the intended use is appropriate
  • Check “host” and “type”
  • Check the “quality” of the presented data/images
  • Appraise whether the available validation meets your needs

Submit a Question

Please complete the form below and a representative will contact you as soon as possible.

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.