Mouse anti-Human LAG3 Monoclonal Antibody | anti-LAG3 antibody

LAG3 Antibody [9F9]

Cat. Num. AAA11017

• Gene Names: LAG3; CD223
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Protein A purified
• Synonyms: LAG3, Antibody; LAG3 Antibody [9F9]; lymphocyte activating 3; LAG-3; CD223; anti-LAG3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 9F9
• Purity/Purification: Protein A purified
• Form/Format: Liquid; Supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 525

• Applicable Applications for anti-LAG3 antibody: ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Application Notes: ELISA starting at 0.25 ug/mL; ICC start at 1 ug/mL; IF start at 10 ug/mL; IHC start at 5 ug/mL; FC/FACS start at 1 ug/ml.; ; Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.
• Immunogen: LAG-3 antibody was raised against the extracellular domain of human LAG-3
• Conjugate: Unconjugated
• Preparation and Storage: Store at 4 degree C for three months and -20 degree C, stable for up to one year.; As with all antibodies care should be taken to avoid repeated freeze thaw cycles.; Antibodies should not be exposed to prolonged high temperatures.

ELISA

(A sandwich ELISA was performed using the anti-LAG3 mAbs as the capture antibodies for the LAG3 extracellular domain antigen with biotin-labeled Risk-Free anti-LAG3 mAbs as the detection antibodies.)

ELISA

(Titration curve analysis of LAG-3 mAbs to detect recombinant LAG-3 in ELISA at decreasing concentrations.)

FCM (Flow Cytometry)

(Flow cytometry analysis of LAG-3 over expressing HEK293 cells using LAG-3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.)

IHC (Immunohistochemistry)

(Immunohistochemistry of LAG-3 in human lymphoma tissue using LAG-3 Antibody and control mouse IgG (corner box) at 5 μg/ml.)

IF (Immunofluorescence)

(Immunofluorescence of LAG-3 in over human spleen tissue using LAG-3 Antibody at 10 μg/ml.Red: LAG3 Antibody [9F9]Blue: DAPI staining)

IF (Immunofluorescence)

(Immunofluorescence of LAG-3 in over expressing HEK293 cells using LAG-3 Antibody at 2 μg/ml.Green: LAG3 Antibody [9F9]Blue: DAPI staining)

ICC (Immunocytochemistry)

(Immunocytochemistry of LAG-3 in over expressing HEK293 cells using LAG-3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.)

Related Product Information for anti-LAG3 antibody

The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.

Product Categories/Family for anti-LAG3 antibody

Immunology

References

Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216-21. Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619-22. Workman CJ, Wang Y, El Kasmi KC, et al. LAG-3 regulates plasmacytoid dendritic cell homeostasis. J Immunol. 2009; 182:1885-91. Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970-9.

NCBI and Uniprot Product Information

• NCBI GI #: 167614500
• NCBI GeneID: 3902
• NCBI Accession #: NP_002277
• NCBI GenBank Nucleotide #: NM_002286.5
• UniProt Accession #: P18627; Q7Z643; A8K7T9
• Molecular Weight: 39,103 Da
• NCBI Official Full Name: lymphocyte activation gene 3 protein
• NCBI Official Synonym Full Names: lymphocyte activating 3
• NCBI Official Symbol: LAG3
• NCBI Official Synonym Symbols: CD223
• NCBI Protein Information: lymphocyte activation gene 3 protein
• UniProt Protein Name: Lymphocyte activation gene 3 protein
• UniProt Gene Name: LAG3
• UniProt Synonym Gene Names: FDC; LAG-3