Rabbit PGAM5 Polyclonal Antibody | anti-PGAM5 antibody

PGAM5 (CT) Antibody

Cat. Num. AAA11041

• Gene Names: PGAM5; BXLBV68
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: PGAM5 Antibody is affinity chromatography purified via peptide column.
• Synonyms: PGAM5, Antibody; PGAM5 (CT) Antibody; Serine/threonine-protein phosphatase PGAM5 mitochondrial; Bcl-XL-binding protein v68; Phosphoglycerate mutase family member 5; PGAM family member 5, mitochondrial serine/threonine protein phosphatase; anti-PGAM5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: PGAM5 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid; PGAM5 Antibody is supplied in PBS containing 0.02% sodium azide
• Concentration: 1mg/mL (varies by lot)

• Applicable Applications for anti-PGAM5 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 0.5ug/mL; IF: 10-20ug/mL; Antibody validated: Western Blot in human, mouse, and rat samples; Immunofluorescence in human, mouse, and rat samples. All other applications and species not yet tested.
• Immunogen: Anti-PGAM5 antibody (9517) was raised against a peptide corresponding to 14 amino acids near the carboxyl terminus of human PGAM5.; The immunogen is located within the last 50 amino acids of PGAM5.
• Homology: Predicted species reactivity based on immunogen sequence: gorilla (100%); Chimpanzee (100%); monkey (100%).
• Research Area: Signal Transduction
• NCBI Organism: Homo sapiens
• Isoforms: Human PGAM5 has 2 isoforms, including isoform 1 (289aa, 32kD), and isoform 2 (255aa, 28kD). Mouse PGAM5 has 2 isoforms, including isoform 1 (288aa, 32kD) and isoform 2 (287aa, 31.9kD). Rat PGAM5 has one isoform (288aa, 32kD). can detect PGAM5 in human, mouse and rat.
• Preparation and Storage: PGAM5 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Figure 7 Immunofluorescence Validation of PGAM5 in Rat TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PGAM5 with AAA11041 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence)

(Figure 6 Immunofluorescence Validation of PGAM5 in Mouse TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse testis labeling PGAM5 with AAA11041 at 10ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence)

(Figure 5 Immunofluorescence Validation of PGAM5 in Human LiverImmunofluorescent analysis of 4% paraformaldehyde-fixed human liver labeling PGAM5 with AAA11041 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

WB (Western Blot)

(Figure 4 Western Blot Validation in Rat Tissues Loading: 15ug of lysates per lane. Antibodies: PGAM5, AAA11041 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)

WB (Western Blot)

(Figure 3 Western Blot Validation in Mouse Tissues Loading: 15ug of lysates per lane. Antibodies: PGAM5, AAA11041 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)

WB (Western Blot)

(Figure 2 Western Blot Validation in Human Cell Lines Loading: 15ug of lysates per lane. Antibodies: PGAM5, AAA11041 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)

WB (Western Blot)

(Figure 1 Western Blot Validation in Mouse and Rat Cell Lines Loading: 15ug of lysates per lane. Antibodies: PGAM5, AAA11041 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)

Related Product Information for anti-PGAM5 antibody

PGAM5 Antibody: PGAM5 is a central mediator for programmed necrosis induced by TNF and may be a regulator of mitochondrial dynamics. It displays phosphatase activity for serine/threonine residues, dephosphorylates and activates MAP3K5 kinase. It has apparently no phosphoglycerate mutase activity and is a substrate for a KEAP1-dependent ubiquitin ligase complex. It is also contributes to the repression of NFE2L2-dependent gene expression

Product Categories/Family for anti-PGAM5 antibody

Primary Antibodies

References

Lo S. et al. Exp. Cell Res. 2018; 314(8): 1789-1803
Takeda et al. PNAS 2009; 106(30): 12301-12305
Want et al. Cell 2012; 148(1-2); 228-243

NCBI and Uniprot Product Information

• NCBI GI #: 150417955
• NCBI GeneID: 192111
• NCBI Accession #: NP_001164014.1
• NCBI GenBank Nucleotide #: NM_001170543.1
• UniProt Accession #: Q96HS1; Q96JB0; A9LN06; C9IZY7
• Molecular Weight: 32,004 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase PGAM5, mitochondrial isoform 1
• NCBI Official Synonym Full Names: phosphoglycerate mutase family member 5
• NCBI Official Symbol: PGAM5
• NCBI Official Synonym Symbols: BXLBV68
• NCBI Protein Information: serine/threonine-protein phosphatase PGAM5, mitochondrial; Bcl-XL-binding protein v68
• UniProt Protein Name: Serine/threonine-protein phosphatase PGAM5, mitochondrial
• UniProt Gene Name: PGAM5
• UniProt Entry Name: PGAM5_HUMAN