Rabbit NCOA4 Polyclonal Antibody | anti-NCOA4 antibody
NCOA4 (IN) Antibody
IHC (Immunohistochemistry)
(Figure 7: Immunohistochemistry Validation of NCOA4 in Rat lungImmunohistochemical analysis of paraffin-embedded rat tissue using anti-NCOA4 antibody (AAA11046) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IHC (Immunohistchemistry)
(Figure 6: Immunohistochemistry Validation of NCOA4 in Mouse lungImmunohistochemical analysis of paraffin-embedded mouse tissue using anti-NCOA4 antibody (AAA11046) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IHC (Immunohistochemistry)
(Figure 5: Immunohistochemistry Validation of NCOA4 in Human lungImmunohistochemical analysis of paraffin-embedded human mouse tissue using anti-NCOA4 antibody (AAA11046) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IF (Immunofluorescence)
(Figure 4: Immunofluorescence Validation of NCOA4 in Daudi CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling NCOA4 with AAA11046 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
WB (Western Blot)
(Figure 3: Western Blot Validation in Rat TissuesLoading: 15ug of lysates per lane.Antibodies: NCOA4 AAA11046, 1ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 2: Western Blot Validation in Mouse Tissues Loading: 15ug of lysates per lane. Antibodies: NCOA4 AAA11046, 1ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 1: Western Blot Validation in Human Cell LinesLoading: 15ug of lysates per lane. Antibodies: NCOA4 AAA11046, 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
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Mou et al. BMC Cancer. 2021; 21(1):18
