Reactivity
Human
Sequence Length
121
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids
Detection Range
5-1000ng/L
Sensitivity
3.11ng/L
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Related Product Information for VIP elisa kit
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human VIP antibody. VIP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human VIP Antibody is added and binds to VIP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated VIP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human VIP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Vasoactive Intestinal Peptide (also known as VIP) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Vasoactive Intestinal Peptide (also known as VIP) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
19,082 Da
NCBI Official Full Name
vasoactive intestinal peptide, partial
NCBI Official Synonym Full Names
vasoactive intestinal peptide
NCBI Official Symbol
VIP
NCBI Official Synonym Symbols
PHM27
NCBI Protein Information
VIP peptides
UniProt Protein Name
VIP peptides
UniProt Gene Name
VIP
UniProt Synonym Gene Names
VIP