Reactivity
Mouse
Sequence Length
107
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
2ng/L - 600ng/L
Sensitivity
1.06ng/L
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Related Product Information for GAS elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Gastrin (also known as GAS) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse GAS antibody. GAS present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse GAS Antibody is added and binds to GAS in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GAS antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse GAS. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse GAS antibody. GAS present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse GAS Antibody is added and binds to GAS in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GAS antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse GAS. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
11,884 Da
NCBI Official Full Name
gastrin
NCBI Official Synonym Full Names
gastrin
NCBI Official Symbol
GAST
NCBI Protein Information
gastrin
UniProt Protein Name
Gastrin
UniProt Gene Name
GAST
UniProt Synonym Gene Names
GAS; G34