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Application Data
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Mouse CD59 Monoclonal Antibody | anti-CD59 antibody

MOUSE ANTI HUMAN CD59:FITC

Gene Names
CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
Applications
Flow Cytometry, Functional Assay
Synonyms
CD59, Antibody; MOUSE ANTI HUMAN CD59:FITC; anti-CD59 antibody
Ordering
 
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For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
MEM-43
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1 mg/ml (varies by lot)
Sequence Length
128
Applicable Applications for anti-CD59 antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry: Maximum Dilution: Neat
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Application Data

(Staining of KG1 lymphocytes with Mouse anti Human CD59:FITC)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD59: Azide Free)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD59:RPE)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD59:Alexa Fluor488)

Application Data

(Published customer image: The effect of protease treatment on influenza virion associated host proteins. Purified influenza A/WSN/33 virus was either mock treated or subjected to overnight digestion with subtilisin followed by concentration through a sucrose cushion. 10 ug of mock infected cell lysate (lane 1) or influenza infected cell lysate (lane 2) and 2 ug of untreated influenza virions (lane 3) or protease treated influenza virions (lane 4) were then analyzed by western blot with antibodies against the indicated proteins. Numbers to the right are molecular weight markers.From: Shaw ML, Stone KL, Colangelo CM, Gulcicek EE, Palese P (2008) Cellular Proteins in Influenza Virus Particles. PLoS Pathog 4(6): e1000085.)

Application Data

(Staining of human peripheral blood granulocytes with Mouse anti Human CD59:Alexa Fluor 647)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD59)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD59)

Application Data

(Staining of human peripheral blood granulocytes with Mouse anti Human CD59:Biotin)

Related Product Information for anti-CD59 antibody
Mouse anti Human CD59 antibody, clone MEM-43 reacts with a PI-linked glycoprotein, Mw 18-20kDa found on all types of leucocytes including platelets. The antigen is expressed on many haemapoietic and non-haemapoietic cells. MEM-43 has been shown to stimulate NK activity. CD59 is expressed by erythrocytes and therefore staining in whole blood systems requires a high concentration of antibody. The epitope recognized by Mouse anti Human CD59 antibody, clone MEM-43 is lost after reduction, therefore, non-reducing conditions are required for western blotting techniques.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
966
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
14,177 Da
NCBI Official Full Name
CD59 glycoprotein preproprotein
NCBI Official Synonym Full Names
CD59 molecule, complement regulatory protein
NCBI Official Symbol
CD59
NCBI Official Synonym Symbols
1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
NCBI Protein Information
CD59 glycoprotein; 1F5 antigen; 20 kDa homologous restriction factor; CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344); Ly-6-like protein; MEM43 antigen; T cell-activating protein; human leukocyte antigen MIC11; lymphocytic antigen CD59/MEM43; membrane attack complex (MAC) inhibition factor; membrane attack complex inhibition factor; membrane inhibitor of reactive lysis; protectin; surface anitgen recognized by monoclonal antibody 16.3A5
UniProt Protein Name
CD59 glycoprotein
UniProt Gene Name
CD59
UniProt Synonym Gene Names
MIC11; MIN1; MIN2; MIN3; MSK21; HRF-20; HRF20; MAC-IP; MACIF; MIRL
UniProt Entry Name
CD59_HUMAN

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Product Notes

The CD59 cd59 (Catalog #AAA11857) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD59 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. Flow Cytometry: Maximum Dilution: Neat. Researchers should empirically determine the suitability of the CD59 cd59 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD59, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.