Mouse CD172a Monoclonal Antibody | anti-CD172a antibody

MOUSE ANTI RAT CD172a

Cat. Num. AAA11967

• Gene Names: Sirpa; Bit; Ptpns1; SHPS-1
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Synonyms: CD172a, Antibody; MOUSE ANTI RAT CD172a; anti-CD172a antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: OX-41
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 509

• Applicable Applications for anti-CD172a antibody: Immunohistology Frozen, Flow cytometry (FC/FACS), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: 1/100; Maximum Dilution: 1/200; Immunohistology - Paraffin: Application Note: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
• Perservative Stabilisers: 0.09% Sodium Azide; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Rat
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Application Data

(Published clone specific image Alloimmunity-associated cytotoxicity is mediated through the NKG2D receptor. (A) Liver expression of nkg2d on day ten after liver transplantation. (B) Representative NKG2D expression levels in blood NK cells (left) and monocytes (right) of allogeneic (black) and syngeneic (grey) recipients. Isotype was used as control (dashed lines). (C) Sorted blood NK cell cytotoxicity inhibition with anti-NKG2D antibody or with anti-NKp30 antibody. (D) Levels of NKG2D ligand (rae1l, rrlt and irp94) expression in the liver on day ten after transplantation. (E) Levels of NKG2D ligand (rae1l, rrlt and irp94) expression in rat HCC cell lines. (F) Representative level of recombinant NKG2D-Fc binding to rat HCC cells lines. *p)

Application Data

(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. Low power)

Application Data

(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. High power)

Application Data

(Immunofluorescence staining of rat lymphnode cryosection with Mouse anti Rat CD172a antibody , red in A and Mouse anti Rat CD4 , green in B. C is the Merged image with nuclei counter-stained blue using DAPI. Medium power)

Application Data

(Immunofluorescence staining of rat lymphnode cryosection with Mouse anti Rat CD172a antibody , red in A and Mouse anti Rat CD4 , green in B. C is the Merged image with nuclei counter-stained blue using DAPI. Low power)

Application Data

(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. Medium power)

Application Data

(Immunofluorescence staining of rat lymphnode cryosection with Mouse anti Rat CD172a antibody , red in A and Mouse anti Rat CD4 , green in B. C is the Merged image with nuclei counter-stained blue using DAPI. High power)

Related Product Information for anti-CD172a antibody

Mouse anti Rat CD172a antibody, clone OX-41 recognizes rat CD172a, also known as Signal regulatory protein (SIRP). CD172a is expressed selectively by myeloid cells and by neurons. MRC OX-41 binds to a different epitope on SIRP than ED9. It does not block the interaction of CD172a - CD47.

NCBI and Uniprot Product Information

• NCBI GI #: 31543529
• NCBI GeneID: 25528
• NCBI Accession #: NP_037148.2
• NCBI GenBank Nucleotide #: NM_013016.2
• UniProt Accession #: P97710; O08951; O70426; Q9QWI5
• Molecular Weight: 55,691 Da
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type substrate 1
• NCBI Official Synonym Full Names: signal-regulatory protein alpha
• NCBI Official Symbol: Sirpa
• NCBI Official Synonym Symbols: Bit; Ptpns1; SHPS-1
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type substrate 1; Brain immunoglobulin like protein with tyrosine - based activation motifs; CD172 antigen-like family member A; Protein tyrosine phosphatase non-receptor type substrate 1 (SHP substrate 1); Protein tyrosine phosphatase, non-receptor type substrate 1 (SHP substrate 1); brain Ig-like molecule with tyrosine-based activation motifs; inhibitory receptor SHPS-1; macrophage fusion receptor; macrophage membrane protein MFP150; signal-regulatory protein alpha-1; sirp-alpha-1
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type substrate 1
• UniProt Gene Name: Sirpa
• UniProt Synonym Gene Names: Bit; Mfr; Ptpns1; Shps1; Sirp; SHP substrate 1; SHPS-1; Bit; Sirp-alpha-1
• UniProt Entry Name: SHPS1_RAT