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Application Data
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Mouse CD169 Monoclonal Antibody | anti-CD169 antibody

MOUSE ANTI RAT CD169

Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation
Synonyms
CD169, Antibody; MOUSE ANTI RAT CD169; anti-CD169 antibody
Ordering
 
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For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
ED3
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 0.5 mg/ml (varies by lot)
Applicable Applications for anti-CD169 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology - Frozen: Minimum Dilution: 1/50; Maximum Dilution: 1/250
Flow Cytometry: Maximum Dilution: 1/500
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Rat
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Application Data

(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. High power)

Application Data

(Frozen rat spleen stained with Mouse anti Rat CD169 followed by Goat anti Mouse IgG:HRP (Rat Adosorbed, Data)

Application Data

(Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. Medium power)

Application Data

(Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. High power)

Application Data

(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. Medium power)

Application Data

(Published customer image: Systemically administered liposomes home primarily to splenic marginal zone and red pulp macrophages. (a,b) Healthy rats were injected with 5 mg/kg DiI-labeled PCLs and PSLs. Splenic cryosections were stained with CD169 (a, marginal metallophilic and marginal zone macrophages) and CD68 (b, red pulp macrophages). One representative experiment is shown (20x magnification).From: Bogie JF, Jorissen W, Mailleux J, Nijland PG, Zelcer N, Vanmierlo T, Van Horssen J, Stinissen P, Hellings N, Hendriks JJ. Myelin alters the inflammatory phenotype of macrophages by activating PPARs. Acta Neuropathol Commun. 2013 Aug 2;1(1):43.)

Application Data

(Published customer image: Immunostaining of rat spleen. Serial-cut frozen sections were stained with mAbs towards A, rat MCL B, rat CD169 C, rat MHC class II D, human MHC class I (negative control) and visualized with peroxidase-conjugated secondary antibody and DAB substrate. RP: red pulp. PALS: periarteriolar lymphoid sheath. FOLL: follicle. MZ: marginal zone.From: Lobato-Pascual A, Saether PC, Dahle MK, Gaustad P, Dissen E, et al. (2013) Rat Macrophage C-Type Lectin Is an Activating Receptor Expressed by Phagocytic Cells. PLoS ONE 8(2): e57406.)

Application Data

(Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. Low power)

Related Product Information for anti-CD169 antibody
Mouse anti rat CD169 antibody, clone ED3 recognises the rat CD169 cell surface antigen, a 185kD molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicious property of ED3 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the ED3 antigen. In healthy tissue no expression occurs. CD169 is a receptor for glycoconjugates containing sialic acid.

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Product Notes

The CD169 (Catalog #AAA11973) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD169 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Immunohistology - Frozen: Minimum Dilution: 1/50; Maximum Dilution: 1/250 Flow Cytometry: Maximum Dilution: 1/500. Researchers should empirically determine the suitability of the CD169 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD169, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.