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Application Data
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Mouse CD312 Monoclonal Antibody | anti-CD312 antibody

MOUSE ANTI HUMAN CD312:RPE

Gene Names
EMR2; CD312
Applications
Flow Cytometry, Functional Assay
Synonyms
CD312, Antibody; MOUSE ANTI HUMAN CD312:RPE; anti-CD312 antibody
Ordering
 
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For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2A1
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Sequence Length
765
Applicable Applications for anti-CD312 antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Maximum Dilution: Neat
Reconstitution
Reconstitute with 1.0 ml distilled water
Perservative Stabilisers
Immunogen
NIH-3T3 cells stably transfected with EMR2 (EGF1-5) cDNA
Fusion Partners
Target Species
Human
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.

Application Data

(Staining of human peripheral blood granulocytes with Mouse anti Human CD312: Alexa Fluor 488)

Application Data

(Staining of human peripheral blood granulocytes with Mouse anti Human CD312: RPE)

Application Data

(Staining of human peripheral blood monocytes with Mouse anti Human CD312: Biotin)

Application Data

(Staining of human peripheral blood monocytes probed with Mouse anti Human CD312 : Alexa Fluor 647)

Application Data

(Staining of human peripheral blood monocytes with Mouse anti Human CD312: FITC)

Application Data

(Staining of human peripheral blood granulocytes with Mouse anti Human CD312)

Related Product Information for anti-CD312 antibody
Mouse anti Human CD312 antibody, clone 2A1 recognizes human EMR2, a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family of proteins, which is closely related to CD97. EMR2, also known as CD312, is predominantly expressed on myeloid dendritic cells, monocytes and tissue macrophages. Various isoforms of EMR2 have been documented. The ligand for the largest isoform of EMR2 has recently been identified as chrondroitin sulphate, which binds to the fourth EGF-like module of EMR2. Clone 2A1 specifically recognizes the stalk region of EMR2.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
84,079 Da
NCBI Official Full Name
EGF-like module-containing mucin-like hormone receptor-like 2 isoform h
NCBI Official Synonym Full Names
egf-like module containing, mucin-like, hormone receptor-like 2
NCBI Official Symbol
EMR2
NCBI Official Synonym Symbols
CD312
NCBI Protein Information
EGF-like module-containing mucin-like hormone receptor-like 2
UniProt Protein Name
EGF-like module-containing mucin-like hormone receptor-like 2
UniProt Gene Name
EMR2
UniProt Entry Name
EMR2_HUMAN

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Product Notes

The CD312 emr2 (Catalog #AAA12143) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD312 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Maximum Dilution: Neat. Researchers should empirically determine the suitability of the CD312 emr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD312, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.