Rat Thyroxine (T4) ELISA Kit | T4 elisa kit
Rat Thyroxine (T4) ELISA Kit
Reactivity
Rat
Synonyms
Thyroxine (T4); N/A; Rat Thyroxine (T4) ELISA Kit; T4 elisa kit
Reactivity
Rat
Sequence Length
525
Assay Type
Sandwich
Detection Range
200 nmol/L-3.12 nmol/L
Sensitivity
Up to 0.6 nmol/L
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for T4 elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rat T4 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to thewells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,986 Da
NCBI Official Full Name
T4
NCBI Official Symbol
THVgp004
NCBI Protein Information
T4
UniProt Protein Name
T4
UniProt Entry Name
Q91TV9_TUHV1
