Porcine Surfactant Associated Protein B (SP-B) ELISA Kit | SP-B elisa kit

Porcine Surfactant Associated Protein B (SP-B) ELISA Kit

Cat. Num. AAA13037

• Gene Names: Sftpb; Sp-b
• Reactivity: Porcine
• Synonyms: Surfactant Associated Protein B (SP-B); N/A; Porcine Surfactant Associated Protein B (SP-B) ELISA Kit; SP-B elisa kit

• Reactivity: Porcine
• Specificity: No cross-reaction with other factors.
• Sequence Length: 376
• Assay Type: Sandwich
• Detection Range: 100 ng/mL-1.56 ng/mL
• Sensitivity: Up to 0.5 ng/mL

• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for SP-B elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Porcine SP-B monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 149036401
• NCBI GeneID: 192155
• Molecular Weight: 41,590 Da
• NCBI Official Full Name: surfactant associated protein B
• NCBI Official Synonym Full Names: surfactant protein B
• NCBI Official Symbol: Sftpb
• NCBI Official Synonym Symbols: Sp-b
• NCBI Protein Information: pulmonary surfactant-associated protein B; surfactant associated protein B; surfactant pulmonary-associated protein B; surfactant, pulmonary-associated protein B; pulmonary surfactant-associated proteolipid SPL(Phe)
• UniProt Protein Name: Pulmonary surfactant-associated protein B
• UniProt Gene Name: Sftpb
• UniProt Synonym Gene Names: Sftp3; SP-B
• UniProt Entry Name: PSPB_RAT