Reactivity
Rat
Sequence Length
601
Assay Type
Sandwich
Samples
Rat serum, plasma, or cell culture supernatant.
Detection Range
20 ng/mL-0.312 ng/mL
Sensitivity
Up to 0.06 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for ANA elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antigen Sandwich ELISA technique. The pre-coated antigen is a related-Rat ANA antigen, while the detection antigen is another antigen. Samples are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
64,173 Da
NCBI Official Full Name
AT-rich interactive domain-containing protein 3A
NCBI Official Synonym Full Names
AT rich interactive domain 3A (BRIGHT-like)
NCBI Official Symbol
Arid3a
NCBI Official Synonym Symbols
Dri1; Dril1; Bright
NCBI Protein Information
AT-rich interactive domain-containing protein 3A; dead ringer homolog 1; dead ringer-like protein 1; ARID domain-containing protein 3A; B-cell regulator of IgH transcription; AT rich interactive domain 3A (Bright like)
UniProt Protein Name
AT-rich interactive domain-containing protein 3A
UniProt Gene Name
Arid3a
UniProt Synonym Gene Names
Dri1; Dril1; ARID domain-containing protein 3A; Bright
UniProt Entry Name
ARI3A_MOUSE