Human anti-nucleosome antibody IgG (AnuA-IgG) ELISA Kit | AnuA-IgG elisa kit
Human anti-nucleosome antibody IgG (AnuA-IgG) ELISA Kit
Reactivity
Human
Synonyms
anti-nucleosome antibody IgG (AnuA-IgG); N/A; Human anti-nucleosome antibody IgG (AnuA-IgG) ELISA Kit; AnuA-IgG elisa kit
Reactivity
Human
Assay Type
Sandwich
Samples
Human serum, plasma, or cell culture supernatant.
Detection Range
200 ng/mL-3.12 ng/mL
Sensitivity
Up to 0.6 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for AnuA-IgG elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antigen Sandwich ELISA technique. The pre-coated antigen is a related-Human AnuA-IgG antigen, while the detection antigen is another antigen. Samples are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
