Reactivity
Pig
Specificity
This assay has high sensitivity and excellent specificity for detection of Pig HA. No significant cross-reactivity or interference between Pig HA and analogues was observed.
Assay Type
Quantitative Sandwich
Samples
Serum, plasma and other biological fluids
Detection Range
7.8 ng/mL - 500 ng/mL
Sensitivity
3.9 ng/mL (mean of 6 independent assays)
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for HA elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate HA in Pig serum, plasma. An antibody specific for HA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HA present is bound by the immobilized antibody. After removing any unbound substances, a biotin- conjugated antibody specific for HA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Intended Uses: For the quantitative detection of Pig Hyaluronic acid (HA) concentration in serum, plasma and other biological fluids.
Intended Uses: For the quantitative detection of Pig Hyaluronic acid (HA) concentration in serum, plasma and other biological fluids.