Mouse Ceramide kinase ELISA Kit | Cerk elisa kit
Mouse Ceramide kinase ELISA Kit
Gene Names
Cerk; AI848610; mKIAA1646; D330016D08Rik
Reactivity
Mouse
Synonyms
Ceramide kinase; N/A; Mouse Ceramide kinase ELISA Kit; mCERK; Acylsphingosine kinase; Cerk; 2.7.1.138; Cerk elisa kit
Reactivity
Mouse
Specificity
Recombinant and natural Mouse Ceramide kinase,mCERK
Sequence Length
531
Samples
Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
Detection Range
78-5000 pg/mL
Intra-assay Precision
Intra-Assay CV: <=5.9%
Inter-assay Precision
Inter-Assay CV: <=9.1%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Standard Curve (Sample)
Related Product Information for Cerk elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,843 Da
NCBI Official Full Name
ceramide kinase
NCBI Official Synonym Full Names
ceramide kinase
NCBI Official Symbol
Cerk
NCBI Official Synonym Symbols
AI848610; mKIAA1646; D330016D08Rik
NCBI Protein Information
ceramide kinase
UniProt Protein Name
Ceramide kinase
UniProt Gene Name
Cerk
UniProt Synonym Gene Names
mCERK
UniProt Entry Name
CERK1_MOUSE
