COVID 19 high sensitivity Spike S1 IgG Coronavirus ELISA Kit | COVID-19 elisa kit
High-sensitive SARS-CoV-2 S1 IgG ELISA Kit
Synonyms
COVID 19 high sensitivity Spike S1 IgG Coronavirus; N/A; High-sensitive SARS-CoV-2 S1 IgG ELISA Kit; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike S1 IgG; COVID-19 elisa kit
Specificity
We have tested this assay in blood samples of 60 well-characterized COVID-19 patients, and 58 of 60 patients were anti-SARS-CoV-2 S1 IgG positive. 20 blood samples from healthy individuals were tested, and none of them were positive. The sensitivity of this assay is >= 96%, and the specificity of this assay is >=98%.
Samples
Serum, plasma or other specimen
Intra-assay Precision
Three different known levels of positive control were spiked into sample buffer as test samples. All samples were tested on the same plate to evaluate intra-assay precision of the kit. The Intra- assay precision of this kit is less than 5%.
Inter-assay Precision
Three different known levels of control were spiked into sample buffer as test samples. All samples were tested in 3 separate assays to evaluate intra-assay precision of the kit. Inter-assay precision of this kit is less than 7%.
Standard Curve (Sample)
Related Product Information for COVID-19 elisa kit
Background: SARS-CoV-2 is an enveloped virus with a positive-sense RNA genome and a nucleocapsid of helical symmetry. The SARS-CoV-2 entry into host cells is mediated by the transmembrane spike (S) glycoprotein.
Principle of the Assay: 96-well plates are coated with SARS-CoV-2 S1 protein that captures IgG antibodies against SARS- CoV-2 S1 protein in the sample. After washing away unbound materials, captured IgG against SARS-CoV-2 S1 protein is detected by anti-human IgG polyclonal antibodies conjugated with horse radish peroxidase (HRP). After washing step, the chromogenic substrate 3,3',5,5'- tetramethylbenzidine (TMB) is added. Color reaction is stopped by 2M H2SO4. The amount of anti- SARS- CoV-2 S1 antibodies captured inside the wells is proportional to the color density generated in the coupled oxidation-reduction reaction. The unknown sample concentration can be interpolated from a standard curve generated by Human anti-S1 monoclonal antibody (mAb).
Intended Uses: SARS-CoV-2 S1 protein IgG ELISA Kit is a highly sensitive and specific immunoassay for the precision detection and quantitative measurement of IgG class antibodies against the spike S1 protein of SARS-CoV-2 virus in human blood samples in vitro.
Principle of the Assay: 96-well plates are coated with SARS-CoV-2 S1 protein that captures IgG antibodies against SARS- CoV-2 S1 protein in the sample. After washing away unbound materials, captured IgG against SARS-CoV-2 S1 protein is detected by anti-human IgG polyclonal antibodies conjugated with horse radish peroxidase (HRP). After washing step, the chromogenic substrate 3,3',5,5'- tetramethylbenzidine (TMB) is added. Color reaction is stopped by 2M H2SO4. The amount of anti- SARS- CoV-2 S1 antibodies captured inside the wells is proportional to the color density generated in the coupled oxidation-reduction reaction. The unknown sample concentration can be interpolated from a standard curve generated by Human anti-S1 monoclonal antibody (mAb).
Intended Uses: SARS-CoV-2 S1 protein IgG ELISA Kit is a highly sensitive and specific immunoassay for the precision detection and quantitative measurement of IgG class antibodies against the spike S1 protein of SARS-CoV-2 virus in human blood samples in vitro.
