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Standard Curve (Sample)

Bovine adrenocorticotropic hormone (ACTH) ELISA Kit | ACTH elisa kit

Bovine adrenocorticotropic hormone, ACTH ELISA Kit

Reactivity
Bovine
Synonyms
adrenocorticotropic hormone (ACTH); N/A; Bovine adrenocorticotropic hormone, ACTH ELISA Kit; Bovine adrenocorticotropic hormone (ACTH) ELISA kit; ACTH elisa kit
Ordering
 
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For Research Use Only!
Reactivity
Bovine
Specificity
This assay has high sensitivity and excellent specificity for detection of bovine ACTH. No significant cross-reactivity or interference between bovine ACTH and analogues was observed.
Sequence Length
41
Samples
Serum, plasma
Assay Type
Competitive
Detection Range
0.12 pg/ml -500 pg/ml.
Sensitivity
The minimum detectable dose of bovine ACTH is typically less than 0.12 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest bovine ACTH concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for ACTH elisa kit
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are added to the appropriate microtiter plate wells with antibody specific for ACTH and Horseradish Peroxidase (HRP) conjugated goat-anti-rabbit antibody. The competitive inhibition reaction is launched between with pre-coated ACTH and ACTH in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of ACTH in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI Official Full Name
ACTH, partial

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Product Notes

The Bovine ACTH (Catalog #AAA15102) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA15102 ELISA Kit recognizes Bovine ACTH. It is sometimes possible for the material contained within the vial of "adrenocorticotropic hormone (ACTH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.