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Standard Curve (Sample)

Rat factor B, BF ELISA Kit | BF elisa kit

Rat factor B, BF ELISA Kit

Gene Names
CFB; BF; FB; BFD; GBG; CFAB; CFBD; PBF2; AHUS4; FBI12; H2-Bf; ARMD14
Reactivity
Rat
Synonyms
factor B, BF; N/A; Rat factor B, BF ELISA Kit; Rat factor B; BF ELISA Kit; factor B; BF; BF elisa kit
Ordering
 
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For Research Use Only!
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat BF. No significant cross-reactivity or interference between rat BF and analogues was observed.
Sequence Length
621
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
1.56 ng/ml -100 ng/ml.
Sensitivity
The minimum detectable dose of rat BF is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for BF elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BF bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
629
UniProt Accession #
Molecular Weight
68,872 Da
NCBI Official Full Name
factor B
NCBI Official Synonym Full Names
complement factor B
NCBI Official Symbol
CFB
NCBI Official Synonym Symbols
BF; FB; BFD; GBG; CFAB; CFBD; PBF2; AHUS4; FBI12; H2-Bf; ARMD14
NCBI Protein Information
complement factor B; C3 proactivator; C3/C5 convertase; C3 proaccelerator; properdin factor B; B-factor, properdin; glycine-rich beta glycoprotein; glycine-rich beta-glycoprotein
UniProt Protein Name
Complement factor B
UniProt Gene Name
CFB
UniProt Synonym Gene Names
BF; BFD; GBG
UniProt Entry Name
CFAB_HUMAN

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Product Notes

The Rat BF cfb (Catalog #AAA15205) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA15205 ELISA Kit recognizes Rat BF. It is sometimes possible for the material contained within the vial of "factor B, BF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.