Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat cortisol. No significant cross-reactivity or interference between rat cortisol and analogues was observed.
Assay Type
Competitive
Samples
Serum, plasma
Detection Range
3.12 ng/ml-200 ng/ml
Sensitivity
< 1.56 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for COR elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are added to the appropriate microtiter plate wells with antibody specific for cortisol and Horseradish Peroxidase (HRP) conjugated goat-anti-rabbit antibody. The competitive inhibition reaction is launched between with pre-coated cortisol and cortisol in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of cortisol in the sample. The color development is stopped and the intensity of the color is measured.