Rat platelet factor 4 ELISA Kit | PF4 elisa kit
Rat Platelet Factor 4, PF-4 ELISA Kit
Reactivity
Rat
Synonyms
platelet factor 4; N/A; Rat Platelet Factor 4, PF-4 ELISA Kit; Rat Platelet Factor 4; PF-4 ELISA Kit; CXCL4; MGC138298; SCYB4; chemokine (C-X-C motif) ligand 4; platelet factor 4 (chemokine (C-X-C motif) ligand 4) ; PF4 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat PF-4. No significant cross-reactivity or interference between rat PF-4 and analogues was observed.
Sequence Length
30
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, tissue homogenates
Detection Range
0.156 ug/ml -10 ug/ml
Sensitivity
< 0.039 ug/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Related Product Information for PF4 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PF-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PF-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PF-4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PF-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
