Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse testosterone. No significant cross-reactivity or interference between mouse testosterone and analogues was observed.
Assay Type
Competitive
Samples
Serum, plasma, tissue homogenates
Detection Range
1 ng/ml-15 ng/ml
Sensitivity
< 0.5 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for T elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated testosterone antibody. The competitive inhibition reaction is launched between testosterone (Standards or samples) and pre-coated testosterone with HRP labeled testosterone antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of testosterone in the sample. The color development is stopped and the intensity of the color is measured.