Pig interleukin 10 ELISA Kit | IL10 elisa kit
Pig interleukin 10, IL-10 ELISA Kit
Gene Names
IL10; CSIF; IL-10
Reactivity
Pig
Synonyms
interleukin 10; N/A; Pig interleukin 10, IL-10 ELISA Kit; Pig interleukin 10; IL-10 ELISA Kit; CSIF; IL-10; IL10A; MGC126450; MGC126451; TGIF; cytokine synthesis inhibitory factor; IL10 elisa kit
Reactivity
Pig
Specificity
This assay has high sensitivity and excellent specificity for detection of pig IL-10. No significant cross-reactivity or interference between pig IL-10 and analogues was observed.
Sequence Length
175
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, tissue homogenates
Detection Range
6.25 pg/ml-400 pg/ml
Sensitivity
< 1.56 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for IL10 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19,934 Da
NCBI Official Full Name
interleukin-10
NCBI Official Symbol
IL10
NCBI Official Synonym Symbols
CSIF; IL-10
NCBI Protein Information
interleukin-10; cytokine synthesis inhibitory factor
UniProt Protein Name
Interleukin-10
UniProt Gene Name
IL10
UniProt Synonym Gene Names
IL-10; CSIF
UniProt Entry Name
IL10_PIG