Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse LZM. No significant cross-reactivity or interference between mouse LZM and analogues was observed.
Sequence Length
148
Samples
Serum, plasma and tissue homogenates
Assay Type
Sandwich
Detection Range
0.78 ng/ml -50 ng/ml.
Sensitivity
The minimum detectable dose of mouse LZM is typically less than 0.195 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for LZM elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LZM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LZM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LZM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LZM bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
16,537 Da
NCBI Official Full Name
lysozyme (EC 3.2.1.17)
NCBI Official Synonym Full Names
lysozyme
NCBI Official Symbol
LYZ
NCBI Official Synonym Symbols
LZM
NCBI Protein Information
lysozyme C; c-type lysozyme; 1,4-beta-N-acetylmuramidase C
UniProt Protein Name
Lysozyme C
UniProt Gene Name
LYZ
UniProt Synonym Gene Names
LZM
UniProt Entry Name
LYSC_HUMAN