Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat C4. No significant cross-reactivity or interference between rat C4 and analogues was observed.
Sequence Length
1665
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, tissue homogenates
Detection Range
12.5 ng/ml-800 ng/ml
Sensitivity
< 3.12 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for C4 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
complement 4
NCBI Official Synonym Full Names
complement 4
NCBI Official Symbol
C4
NCBI Official Synonym Symbols
C4-2
NCBI Protein Information
complement 4; complement C4; MHC-linked complement C4