Human Testosterone ELISA Kit | T elisa kit
Human Testosterone, T ELISA Kit
Reactivity
Human
Synonyms
Testosterone; N/A; Human Testosterone, T ELISA Kit; Human Testoterone; T ELISA Kit; Testoterone; T; T elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human testosterone. No significant cross-reactivity or interference between human testosterone and analogues was observed.
Assay Type
Competitive
Samples
Serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.2 ng/ml-80 ng/ml
Sensitivity
< 0.1 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<15%. Three positive samples were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<15%. Three positive samples were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Related Product Information for T elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for testosterone and Horseradish Peroxidase (HRP) conjugated testosterone. The competitive inhibition reaction is launched between with HRP labeled testosterone and unlabeled testosterone with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of testosterone in the sample. The color development is stopped and the intensity of the color is measured.
