Biotin elisa kit

Biotin ELISA Kit

Cat. Num. AAA15819

• Synonyms: Biotin ELISA Kit; N/A; Biotin; Biotin elisa kit

• Specificity: This assay has high sensitivity and excellent specificity for detection of biotin. No significant cross-reactivity or interference between biotin and analogues was observed.
• Sequence Length: 320
• Assay Type: Competitive
• Samples: Serum, plasma from human, mouse and other species
• Detection Range: 12.5 pg/ml-200 pg/ml
• Sensitivity: < 4.986pg/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for Biotin elisa kit

Principle of the Assay: This assay employs the direct competitive inhibition enzyme immunoassay technique. The microplate was pre-coated with Avidin. Add Biotin (Standards or samples) to the well, and then add HRP-conjugated Biotin. A competitive inhibition reaction is launched between Biotin (Standards or samples) and HRP conjugated Biotin with the Avidin. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of Biotin bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 320088538
• UniProt Accession #: E9A979
• Molecular Weight: 35,396 Da
• NCBI Official Full Name: biotin
• UniProt Protein Name: Biotin
• UniProt Gene Name: birA
• UniProt Synonym Gene Names: SENTW_4215
• UniProt Entry Name: E9A979_SALET