Mouse Growth Differentiation Factor 3 ELISA Kit | GDF3 elisa kit

Mouse Growth Differentiation Factor 3 ELISA Kit

Cat. Num. AAA16075

• Gene Names: GDF3; KFS3; MCOP7; MCOPCB6
• Reactivity: Mouse
• Synonyms: Growth Differentiation Factor 3; N/A; Mouse Growth Differentiation Factor 3 ELISA Kit; GDF3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of GDF3. No significant cross-reactivity or interference between GDF3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GDF3 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 pg/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for GDF3 elisa kit

Principle of the Assay: GDF3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GDF3 antibody and an GDF3-HRP conjugate. The assay sample and buffer are incubated together with GDF3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GDF3 concentration since GDF3 from samples and GDF3-HRP conjugate compete for the anti-GDF3 antibody binding site. Since the number of sites is limited, as more sites are occupied by GDF3 from the sample, fewer sites are left to bind GDF3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GDF3 concentration in each sample is interpolated from this standard curve.

Intended Uses: This GDF3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse GDF3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for GDF3 elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 10190670
• NCBI GeneID: 9573
• NCBI Accession #: NP_065685.1
• NCBI GenBank Nucleotide #: NM_020634.1
• Molecular Weight: 41,387 Da
• NCBI Official Full Name: growth/differentiation factor 3
• NCBI Official Synonym Full Names: growth differentiation factor 3
• NCBI Official Symbol: GDF3
• NCBI Official Synonym Symbols: KFS3; MCOP7; MCOPCB6
• NCBI Protein Information: growth/differentiation factor 3; GDF-3
• UniProt Protein Name: Growth/differentiation factor 3
• UniProt Gene Name: GDF3
• UniProt Synonym Gene Names: UNQ222/PRO248; GDF-3
• UniProt Entry Name: GDF3_HUMAN