Rabbit Platelet Factor 4 ELISA Kit | PF4 elisa kit

Rabbit Platelet Factor 4 ELISA Kit

Cat. Num. AAA16130

• Gene Names: PF4; PF-4; CXCL4; SCYB4
• Reactivity: Rabbit
• Synonyms: Platelet Factor 4; N/A; Rabbit Platelet Factor 4 ELISA Kit; PF4 elisa kit

• Reactivity: Rabbit
• Specificity: This assay has high sensitivity and excellent specificity for detection of PF-4. No significant cross-reactivity or interference between PF-4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PF-4 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive or Sandwich
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Highest Standards: 25ng/mL
• Concentration of Standards: Concentration of Standards
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for PF4 elisa kit

Principle of the Assay: PF-4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PF-4 antibody and an PF-4-HRP conjugate. The assay sample and buffer are incubated together with PF-4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PF-4 concentration since PF-4 from samples and PF-4-HRP conjugate compete for the anti-PF-4 antibody binding site. Since the number of sites is limited, as more sites are occupied by PF-4 from the sample, fewer sites are left to bind PF-4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PF-4 concentration in each sample is interpolated from this standard curve.

Intended Uses: This PF-4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit PF-4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for PF4 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 4505733
• NCBI GeneID: 5196
• NCBI Accession #: NP_002610.1
• NCBI GenBank Nucleotide #: NM_002619.3
• Molecular Weight: 10,845 Da
• NCBI Official Full Name: platelet factor 4
• NCBI Official Synonym Full Names: platelet factor 4
• NCBI Official Symbol: PF4
• NCBI Official Synonym Symbols: PF-4; CXCL4; SCYB4
• NCBI Protein Information: platelet factor 4; iroplact; oncostatin-A; C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4
• UniProt Protein Name: Platelet factor 4
• UniProt Gene Name: PF4
• UniProt Synonym Gene Names: CXCL4; SCYB4; PF-4
• UniProt Entry Name: PLF4_HUMAN