Rabbit Cardiac Troponin T ELISA Kit | cTnT elisa kit

Rabbit Cardiac Troponin T ELISA Kit

Cat. Num. AAA16148

• Gene Names: TNNT2; CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6
• Reactivity: Rabbit
• Synonyms: Cardiac Troponin T; N/A; Rabbit Cardiac Troponin T ELISA Kit; cTnT elisa kit

• Reactivity: Rabbit
• Specificity: This assay has high sensitivity and excellent specificity for detection of cTnT. No significant cross-reactivity or interference between cTnT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between cTnT and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 pg/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for cTnT elisa kit

Principle of the Assay: cTnT ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-cTnT antibody and an cTnT-HRP conjugate. The assay sample and buffer are incubated together with cTnT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the cTnT concentration since cTnT from samples and cTnT-HRP conjugate compete for the anti-cTnT antibody binding site. Since the number of sites is limited, as more sites are occupied by cTnT from the sample, fewer sites are left to bind cTnT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cTnT concentration in each sample is interpolated from this standard curve.

Intended Uses: This cTnT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit cTnT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for cTnT elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 15290516
• NCBI GeneID: 7139
• Molecular Weight: 30,681 Da
• NCBI Official Full Name: cardiac troponin T
• NCBI Official Synonym Full Names: troponin T type 2 (cardiac)
• NCBI Official Symbol: TNNT2
• NCBI Official Synonym Symbols: CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6
• NCBI Protein Information: troponin T, cardiac muscle; troponin T2, cardiac; cardiomyopathy, hypertrophic 2; cardiomyopathy, dilated 1D (autosomal dominant)
• UniProt Protein Name: Troponin T, cardiac muscle
• UniProt Gene Name: TNNT2
• UniProt Synonym Gene Names: TnTc; cTnT
• UniProt Entry Name: TNNT2_HUMAN