Rat Reduced glutathione ELISA Kit | GSH elisa kit
Rat Reduced glutathione ELISA Kit
Reactivity
Rat
Synonyms
Reduced glutathione; N/A; Rat Reduced glutathione ELISA Kit; GSH elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of RGLU. No significant cross-reactivity or interference between RGLU and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RGLU and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
0.1 ug/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for GSH elisa kit
Principle of the Assay: RGLU ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-RGLU antibody and an RGLU-HRP conjugate. The assay sample and buffer are incubated together with RGLU-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RGLU concentration since RGLU from samples and RGLU-HRP conjugate compete for the anti-RGLU antibody binding site. Since the number of sites is limited, as more sites are occupied by RGLU from the sample, fewer sites are left to bind RGLU-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RGLU concentration in each sample is interpolated from this standard curve.
Intended Uses: This RGLU ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat RGLU. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This RGLU ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat RGLU. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Product Categories/Family for GSH elisa kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
24,129 Da
NCBI Official Full Name
Gsh, partial
UniProt Protein Name
Gsh
UniProt Entry Name
A6YB91_PLADU
