Mouse Collagen Type I ELISA Kit | COL1 elisa kit

Mouse Collagen Type I ELISA Kit

Cat. Num. AAA16214

• Reactivity: Mouse
• Synonyms: Collagen Type I; N/A; Mouse Collagen Type I ELISA Kit; COL1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of COL1. No significant cross-reactivity or interference between COL1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between COL1 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ug/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for COL1 elisa kit

Principle of the Assay: COL1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-COL1 antibody and an COL1-HRP conjugate. The assay sample and buffer are incubated together with COL1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COL1 concentration since COL1 from samples and COL1-HRP conjugate compete for the anti-COL1 antibody binding site. Since the number of sites is limited, as more sites are occupied by COL1 from the sample, fewer sites are left to bind COL1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COL1 concentration in each sample is interpolated from this standard curve.

Intended Uses: This COL1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse COL1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for COL1 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 194244922
• Molecular Weight: 37,852 Da
• NCBI Official Full Name: COL1, partial
• UniProt Protein Name: COL1
• UniProt Gene Name: COL1
• UniProt Entry Name: B4YVJ5_BRANI