Mouse Ferritin ELISA Kit | FE elisa kit

Mouse Ferritin ELISA Kit

Cat. Num. AAA16287

• Gene Names: FTH1; FHC; FTH; HFE5; PLIF; FTHL6; PIG15
• Reactivity: Mouse
• Synonyms: Ferritin; N/A; Mouse Ferritin ELISA Kit; FE elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of FE. No significant cross-reactivity or interference between FE and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FE and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for FE elisa kit

Principle of the Assay: FE ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FE antibody and an FE-HRP conjugate. The assay sample and buffer are incubated together with FE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FE concentration since FE from samples and FE-HRP conjugate compete for the anti-FE antibody binding site. Since the number of sites is limited, as more sites are occupied by FE from the sample, fewer sites are left to bind FE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FE concentration in each sample is interpolated from this standard curve.

Intended Uses: This FE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse FE. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for FE elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 306744
• NCBI GeneID: 2495
• Molecular Weight: 21,226 Da
• NCBI Official Full Name: ferritin
• NCBI Official Synonym Full Names: ferritin, heavy polypeptide 1
• NCBI Official Symbol: FTH1
• NCBI Official Synonym Symbols: FHC; FTH; HFE5; PLIF; FTHL6; PIG15
• NCBI Protein Information: ferritin heavy chain; apoferritin; ferritin H subunit; placenta immunoregulatory factor; proliferation-inducing protein 15; cell proliferation-inducing gene 15 protein
• UniProt Protein Name: Ferritin heavy chain
• UniProt Gene Name: FTH1
• UniProt Synonym Gene Names: FTH; FTHL6; OK/SW-cl.84; PIG15; Ferritin H subunit
• UniProt Entry Name: FRIH_HUMAN