Mouse Procollagen Type III C-Terminal Propeptide ELISA Kit | P III CP elisa kit
Mouse Procollagen Type III C-Terminal Propeptide ELISA Kit
Reactivity
Mouse
Synonyms
Procollagen Type III C-Terminal Propeptide; N/A; Mouse Procollagen Type III C-Terminal Propeptide ELISA Kit; P III CP elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of PIIICP. No significant cross-reactivity or interference between PIIICP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PIIICP and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for P III CP elisa kit
Principle of the Assay: PIIICP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PIIICP antibody and an PIIICP-HRP conjugate. The assay sample and buffer are incubated together with PIIICP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PIIICP concentration since PIIICP from samples and PIIICP-HRP conjugate compete for the anti-PIIICP antibody binding site. Since the number of sites is limited, as more sites are occupied by PIIICP from the sample, fewer sites are left to bind PIIICP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PIIICP concentration in each sample is interpolated from this standard curve.
Intended Uses: This PIIICP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PIIICP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This PIIICP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PIIICP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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