Guinea Pig Interleukin 10 ELISA Kit | IL-10 elisa kit
Guinea pig Interleukin 10 ELISA Kit
Reactivity
Guinea Pig
Synonyms
Interleukin 10; N/A; Guinea pig Interleukin 10 ELISA Kit; IL-10 elisa kit
Reactivity
Guinea Pig
Specificity
This assay has high sensitivity and excellent specificity for detection of IL-10. No significant cross-reactivity or interference between IL-10 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-10 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive or Sandwich
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for IL-10 elisa kit
Intended Uses: This IL-10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig IL-10. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: IL-10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IL-10 antibody and an IL-10-HRP conjugate. The assay sample and buffer are incubated together with IL-10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-10 concentration since IL-10 from samples and IL-10-HRP conjugate compete for the anti-IL-10 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-10 from the sample, fewer sites are left to bind IL-10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-10 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: IL-10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IL-10 antibody and an IL-10-HRP conjugate. The assay sample and buffer are incubated together with IL-10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-10 concentration since IL-10 from samples and IL-10-HRP conjugate compete for the anti-IL-10 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-10 from the sample, fewer sites are left to bind IL-10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-10 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for IL-10 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
IL-10
NCBI Official Symbol
SPPV_03
NCBI Protein Information
IL-10
