Mouse Progesterone ELISA Kit | PROG elisa kit
Mouse Progesterone ELISA Kit
Reactivity
Mouse
Synonyms
Progesterone; N/A; Mouse Progesterone ELISA Kit; PROG elisa kit
Reactivity
Mouse
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for PROG elisa kit
Intended Uses: This PROG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PROG. This ELISA kit for research use only!
Principle of the Assay: PROG ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PROG antibody and an PROG-HRP conjugate. The assay sample and buffer are incubated together with PROG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PROG concentration since PROG from samples and PROG-HRP conjugate compete for the anti-PROG antibody binding site. Since the number of sites is limited, as more sites are occupied by PROG from the sample, fewer sites are left to bind PROG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PROG concentration in each sample is interpolated from this standard curve.
Principle of the Assay: PROG ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PROG antibody and an PROG-HRP conjugate. The assay sample and buffer are incubated together with PROG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PROG concentration since PROG from samples and PROG-HRP conjugate compete for the anti-PROG antibody binding site. Since the number of sites is limited, as more sites are occupied by PROG from the sample, fewer sites are left to bind PROG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PROG concentration in each sample is interpolated from this standard curve.
